Attenuation of PM2.5-Induced Lung Injury by 4-Phenylbutyric Acid: Maintenance of [Ca2+]i Stability between Endoplasmic Reticulum and Mitochondria

被引:0
|
作者
Ma, Zhenhua [1 ,2 ]
Du, Xiaohui [1 ]
Sun, Yize [1 ]
Jia, Yunna [1 ]
Liang, Xiaojun [2 ]
Gao, Yunhang [1 ]
机构
[1] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
[2] Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750002, Peoples R China
关键词
PM2.5; 4-PBA; intracellular Ca2+; ER stress; mitochondrial damage; pyroptosis; ER STRESS; PM2.5; EXPOSURE; INFLAMMATION;
D O I
10.3390/biom14091135
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fine particulate matter (PM2.5) is a significant cause of respiratory diseases and associated cellular damage. The mechanisms behind this damage have not been fully explained. This study investigated two types of cellular damage (inflammation and pyroptosis) induced by PM2.5, focusing on their relationship with two organelles (the endoplasmic reticulum and mitochondria). Animal models have demonstrated that PM2.5 induces excessive endoplasmic reticulum stress (ER stress), which is a significant cause of lung damage in rats. This was confirmed by pretreatment with an ER stress inhibitor (4-Phenylbutyric acid, 4-PBA). We found that, in vitro, the intracellular Ca2+ ([Ca2+]i) dysregulation induced by PM2.5 in rat alveolar macrophages was associated with ER stress. Changes in mitochondria-associated membranes (MAMs) result in abnormal mitochondrial function. This further induced the massive expression of NLRP3 and GSDMD-N, which was detrimental to cell survival. In conclusion, our findings provide valuable insights into the relationship between [Ca2+]i dysregulation, mitochondrial damage, inflammation and pyroptosis under PM2.5-induced ER stress conditions. Their interactions ultimately have an impact on respiratory health.
引用
收藏
页数:18
相关论文
共 13 条
  • [1] Attenuation of endoplasmic reticulum stress using the chemical chaperone 4-phenylbutyric acid prevents cardiac fibrosis induced by isoproterenol
    Ayala, Pedro
    Montenegro, Jose
    Vivar, Raul
    Letelier, Alan
    Aranguiz Urroz, Pablo
    Copaja, Miguel
    Pivet, Deisy
    Humeres, Claudio
    Troncoso, Rodrigo
    Miguel Vicencio, Jose
    Lavandero, Sergio
    Diaz-Araya, Guillermo
    EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2012, 92 (01) : 97 - 104
  • [2] Sodium 4-phenylbutyric acid protects against mice from acetaminophen-induced liver injury through attenuation of endoplasmic reticulum stress
    Kusama, Hiromi
    Kon, Kazuyoshi
    Ikejima, Kenichi
    Uchiyama, Akira
    Arai, Kumiko
    Yamashina, Shunhei
    Watanabe, Sumio
    HEPATOLOGY, 2015, 62 : 326A - 326A
  • [3] 4-phenylbutyric acid attenuates endoplasmic reticulum stress-mediated apoptosis and protects the hepatocytes from intermittent hypoxia-induced injury
    Liu Xin
    Wu Fan
    Du Tingting
    Sun Zuoming
    Zhang Qiang
    Sleep and Breathing, 2019, 23 : 711 - 717
  • [4] 4-phenylbutyric acid attenuates endoplasmic reticulum stress-mediated apoptosis and protects the hepatocytes from intermittent hypoxia-induced injury
    Liu Xin
    Wu Fan
    Du Tingting
    Sun Zuoming
    Zhang Qiang
    SLEEP AND BREATHING, 2019, 23 (02) : 711 - 717
  • [5] Attenuation of endoplasmic reticulum stress using the chemical chaperone 4-phenylbutyric acid prevents cardiac fibrosis induced by isoproterenol (vol 92, pg 97, 2012)
    Ayala, Pedro
    Montenegro, Jose
    Vivar, Raul
    Letelier, Alan
    Urroz, Pablo Aranguiz
    Copaja, Miguel
    Pivet, Deisy
    Humeres, Claudio
    Troncoso, Rodrigo
    Vicencio, Jose Miguel
    Lavandero, Sergio
    Diaz-Araya, Guillermo
    EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2021, 121
  • [6] [Ca2+]i signaling between mitochondria and endoplasmic reticulum in neurons is regulated by microtubules -: From mitochondrial permeability transition pore to Ca2+-induced Ca2+ release
    Mironov, SL
    Ivannikov, MV
    Johansson, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (01) : 715 - 721
  • [7] Inhibition of Endoplasmic Reticulum Stress Using 4-phenylbutyric Acid Ameliorates the Severity of Collagen-induced Arthritis in Mice via Attenuation of Proliferation and Inflammatory Responses of Synovial Fibroblasts
    Yoo, Wan-Hee
    Choi, Yunjung
    Lee, Eun-Gyeong
    Lee, Chang-Hoon
    Lee, Myeung-Su
    ARTHRITIS & RHEUMATOLOGY, 2019, 71
  • [8] Chemical chaperone 4-phenylbutyric acid protects H9c2 cardiomyocytes from ischemia/reperfusion injury by attenuating endoplasmic reticulum stress-induced apoptosis
    Jian, Lian
    Lu, Yuan
    Lu, Shan
    Lu, Chengzhi
    MOLECULAR MEDICINE REPORTS, 2016, 13 (05) : 4386 - 4392
  • [9] 4-Phenylbutyric Acid Attenuates Soybean Glycinin/β-Conglycinin-Induced IPEC-J2 Cells Apoptosis by Regulating the Mitochondria-Associated Endoplasmic Reticulum Membrane and NLRP-3
    Wang, Lei
    Zhang, Daoliang
    Jiang, Benzheng
    Ding, Hongyan
    Feng, Shibin
    Zhao, Chang
    Wang, Xichun
    Wu, Jinjie
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2024, 72 (11) : 5926 - 5934
  • [10] Oxidative stress activates the TRPM2-Ca2+-NLRP3 axis to promote PM2.5-induced lung injury of mice
    Wang, Chunyuan
    Meng, Xianzong
    Meng, Meiling
    Shi, Min
    Sun, Wenping
    Li, Xiaojing
    Zhang, Xiao
    Liu, Ruihao
    Fu, Ying
    Song, Laiyu
    BIOMEDICINE & PHARMACOTHERAPY, 2020, 130