BRAZILIN MEDIATES THE HUMAN PERIODONTAL LIGAMENT STEM CELLS FOR PERIODONTITIS TREATMENT THROUGH THE YAP SIGNALING PATHWAY

Objective: To explore the effects of Brazilin on the Human periodontal ligament stem cells (hPDLSCs) mediating periodontal tissue regeneration in periodontitis and the Yes-associated protein (YAP) pathway for periodontitis. Methods: The anti-inflammatory effects and osteogenic effects of Brazilin in the inflammatory microenvironment were mainly evaluated by polymerase chain reaction (PCR), western blot (WB), staining, and the blocking and deletion of the YAP pathway in vitro by verteporfin (VP) and shRNA to evaluated the effect on the YAP pathway. A rat periodontitis model was established and the therapeutic effects were evaluated by Micro-CT, hematoxylin and eosin (HE) staining, Masson staining, and immunohistochemistry. Results: Brazilin effectively down-regulated the expression of inflammatory factors, showed deeper staining, and showed up-regulation of related osteogenic factors, and inhibition of the YAP pathway is followed by inhibition of the osteogenic effects of Brazilin. Brazilin was effective in reducing inflammation and inhibiting alveolar bone loss in the rat periodontitis model. Conclusion: Brazilin can promote periodontal tissue regeneration in periodontitis through the YAP pathway, providing new ideas for the treatment of clinical periodontitis.