Multi-Tissue Controls and Multiplex Immunocytochemistry in Pulmonary Cytology

被引：0
作者：
Vuorisalo, Antti [1 ,2 ]
Haapaniemi, Teppo [2 ,3 ]
Kholova, Ivana [1 ,2 ]
机构：
[1] Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland
[2] Dept Pathol, Fimlab Labs, Tampere, Finland
[3] Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland
来源：
ACTA CYTOLOGICA | 2024年 / 68卷 / 05期
关键词：
Multi-tissue controls; Multiplex immunocytochemistry; Immunocytochemistry; Pulmonary cytology; IMMUNOHISTOCHEMISTRY; CANCER;
D O I：
10.1159/000540367
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Introduction: The World Health Organization 2021 lung cancer classification highlights the central role of immunohistochemistry (IHC) in diagnostic pathology. Despite traditional IHC being essential, its limitation to one marker per tissue section brings challenges, particularly when facing cytological limitedly sized samples. To overcome these challenges, multiplex immunocytochemistry (mICC) techniques offer the simultaneous detection of multiple markers from a single section. These advances complement the highly complex imaging techniques that enable additional analyses of cellular interactions. Methods: The present study outlines a comprehensive mICC methodology of an automated multiplex immunoperoxidase staining method and multiple tissue hybrid controls for ICC/mICC. Protocols are presented in detail and demonstrate a careful approach to optimizing various markers for diagnostic workup including immunotherapy. Conclusion: Multiplex IHC/ICC emerges as a transformative force in biomedical diagnostics and research. Beyond simultaneous marker detection, it unravels complexities within tissues - unveiling co-localization nuances, deciphering expression patterns, and enhancing understanding of cellular populations. As personalized treatments gain prominence, the study emphasizes the heightened importance of diagnostic tools and sample adequacy. The present methodological study, encapsulating an automated multiplex immunoperoxidase staining method, symbolizes a stride towards precision in pulmonary carcinoma diagnosis. Multi-tissue controls represent a key element in quality assurance in pathology laboratories.
引用
收藏
页码：481 / 493
页数：13
相关论文
共 45 条
[1]   Cell blocks from scraping of cytology smear: Comparison with conventional cell block [J].
Bhatia, Prateek ;
Dey, Pranab ;
Uppal, Radha ;
Shifa, Ruth ;
Srinivasan, Radhika ;
Nijhawan, Raje .
ACTA CYTOLOGICA, 2008, 52 (03) :329-333
[2]   The impact of a pathologist's personality on the interobserver variability and diagnostic accuracy of predictive PD-L1 immunohistochemistry in lung cancer [J].
Butter, Rogier ;
Hondelink, Liesbeth M. ;
van Elswijk, Lisette ;
Blaauwgeers, Johannes L. G. ;
Bloemena, Elisabeth ;
Britstra, Rieneke ;
Bulkmans, Nicole ;
van Gulik, Anna Lena ;
Monkhorst, Kim ;
de Rooij, Mathilda J. ;
Slavujevic-Letic, Ivana ;
Smit, Vincent T. H. B. M. ;
Speel, Ernst-Jan M. ;
Thunnissen, Erik ;
von der Thusen, Jan H. ;
Timens, Wim ;
van de Vijver, Marc J. ;
Yick, David C. Y. ;
Zwinderman, Aeilko H. ;
Cohen, Danielle ;
't Hart, Nils A. ;
Radonic, Teodora .
LUNG CANCER, 2022, 166 :143-149
[3]   Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic [J].
Carvajal-Hausdorf, Daniel E. ;
Schalper, Kurt A. ;
Neumeister, Veronique M. ;
Rimm, David L. .
LABORATORY INVESTIGATION, 2015, 95 (04) :385-396
[4]   Documentation of Immunocytochemistry Controls in the Cytopathologic Literature: A Meta-Analysis of 100 Journal Articles [J].
Colasacco, Carol ;
Mount, Sharon ;
Leiman, Gladwyn .
DIAGNOSTIC CYTOPATHOLOGY, 2011, 39 (04) :245-250
[5]   The PD-1/PD-L1-Checkpoint Restrains T cell Immunity in Tumor-Draining Lymph Nodes [J].
Dammeijer, Floris ;
van Gulijk, Mandy ;
Mulder, Evalyn E. ;
Lukkes, Melanie ;
Klaase, Larissa ;
van den Bosch, Thierry ;
van Nimwegen, Menno ;
Lau, Sai Ping ;
Latupeirissa, Kitty ;
Schetters, Sjoerd ;
van Kooyk, Yvette ;
Boon, Louis ;
Moyaart, Antien ;
Mueller, Yvonne M. ;
Katsikis, Peter D. ;
Eggermont, Alexander M. ;
Vroman, Heleen ;
Stadhouders, Ralph ;
Hendriks, Rudi W. ;
von der Thusen, Jan ;
Grunhagen, Dirk J. ;
Verhoef, Cornelis ;
van Hall, Thorbald ;
Aerts, Joachim G. .
CANCER CELL, 2020, 38 (05) :685-+
[6]   Immunohistochemistry Practices of Cytopathology Laboratories A Survey of Participants in the College of American Pathologists Nongynecologic Cytopathology Education Program [J].
Fischer, Andrew H. ;
Schwartz, Mary R. ;
Moriarty, Ann T. ;
Wilbur, David C. ;
Souers, Rhona ;
Fatheree, Lisa ;
Booth, Christine N. ;
Clayton, Amy C. ;
Kurtyz, Daniel F. I. ;
Padmanabhan, Vijayalakshmi ;
Crothers, Barbara A. .
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2014, 138 (09) :1167-1172
[7]   Principles of Analytic Validation of Immunohistochemical Assays [J].
Goldsmith, Jeffrey D. ;
Troxell, Megan L. ;
Roy-Chowdhuri, Sinchita ;
Colasacco, Carol F. ;
Edgerton, Mary Elizabeth ;
Fitzgibbons, Patrick L. ;
Fulton, Regan ;
Haas, Thomas ;
Kandalaft, Patricia L. ;
Kalicanin, Tanja ;
Lacchetti, Christina ;
Loykasek, Patti ;
Thomas, Nicole E. ;
Swanson, Paul E. ;
Bellizzi, Andrew M. .
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2024, 148 (06) :e111-e153
[8]   Eight-Color Multiplex Immunohistochemistry for Simultaneous Detection of Multiple Immune Checkpoint Molecules within the Tumor Microenvironment [J].
Gorris, Mark A. J. ;
Halilovic, Altuna ;
Rabold, Katrin ;
van Duffelen, Anne ;
Wickramasinghe, Iresha N. ;
Verweij, Dagmar ;
Wortel, Inge M. N. ;
Textor, Johannes C. ;
de Vries, I. Jolanda M. ;
Figdor, Carl G. .
JOURNAL OF IMMUNOLOGY, 2018, 200 (01) :347-354
[9]   Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists [J].
Harms, Paul W. ;
Frankel, Timothy L. ;
Moutafi, Myrto ;
Rao, Arvind ;
Rimm, David L. ;
Taube, Janis M. ;
Thomas, Dafydd ;
Chan, May P. ;
Pantanowitz, Liron .
MODERN PATHOLOGY, 2023, 36 (07)
[10]   Multiplexed Immunohistochemistry for Molecular and Immune Profiling in Lung Cancer-Just About Ready for Prime-Time? [J].
Hofman, Paul ;
Badoual, Cecile ;
Henderson, Fiona ;
Berland, Lea ;
Hamila, Marame ;
Long-Mira, Elodie ;
Lassalle, Sandra ;
Roussel, Helene ;
Hofman, Veronique ;
Tartour, Eric ;
Ilie, Marius .
CANCERS, 2019, 11 (03)
12345