AJUBA promotes the proliferation, invasion and migration of NSCLC cells by activating the ERK/β-catenin pathway

被引:0
作者
Qu, Lianyue [1 ,2 ]
Wang, Fan [1 ,4 ]
Wang, Yuxiang [1 ,5 ]
Li, Zixuan [1 ,3 ]
机构
[1] China Med Univ, Hosp 1, Dept Radiol, Key Lab Diagnost Imaging & Intervent Radiol Liaoni, Shenyang, Peoples R China
[2] China Med Univ, Hosp 1, Dept Pharm, Shenyang, Peoples R China
[3] China Med Univ, Hosp 1, Dept Radiol, Shenyang, Peoples R China
[4] China Med Univ, Hosp 1, Dept Intervent Radiol, Shenyang, Peoples R China
[5] China Med Univ, Hosp 1, Dept Nucl Med, Shenyang, Peoples R China
关键词
AJUBA; NSCLC; beta-Catenin; P-ERK; EPITHELIAL-MESENCHYMAL TRANSITION; LIM PROTEIN AJUBA; UP-REGULATION;
D O I
10.1038/s41598-025-98156-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Accumulating evidence indicates that AJUBA acts as a potential target for new therapeutics to treat cancers. Nevertheless, the role of AJUBA in non-small cell lung cancer (NSCLC) remains unclear. In the current study, immunohistochemistry (IHC) showed that expression of AJUBA was upregulated in 67.55% of NSCLC tumor samples and was associated with tumor size, lymph node metastasis, advanced tumor stage, poor differentiation and poor prognosis. Loss-of-function assays of AJUBA produced by silencing RNA (siAJUBA) significantly inhibited the proliferation, invasion and migration of H1299 and A549 cell lines. Mechanistically, inhibition of extracellular signal-regulated kinases (ERKs) blocked the AJUBA-induced proliferation, invasion and migration of NSCLC cells, and decreased the expression of proteins related to the endothelial-mesenchymal transition (EMT). Silencing of AJUBA repressed tumor growth and led to a decrease in p-ERK, beta-catenin and N-cadherin in vivo. In conclusion,, overexpression of AJUBA facilitates the proliferation and motility of NSCLC cells via the ERK and Wnt/beta-catenin pathways. AJUBA may be useful as a prognostic marker which may provide a promising approach for the treatment of NSCLC.
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页数:12
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