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AJUBA promotes the proliferation, invasion and migration of NSCLC cells by activating the ERK/β-catenin pathway
被引:0
作者:
Qu, Lianyue
[1
,2
]
Wang, Fan
[1
,4
]
Wang, Yuxiang
[1
,5
]
Li, Zixuan
[1
,3
]
机构:
[1] China Med Univ, Hosp 1, Dept Radiol, Key Lab Diagnost Imaging & Intervent Radiol Liaoni, Shenyang, Peoples R China
[2] China Med Univ, Hosp 1, Dept Pharm, Shenyang, Peoples R China
[3] China Med Univ, Hosp 1, Dept Radiol, Shenyang, Peoples R China
[4] China Med Univ, Hosp 1, Dept Intervent Radiol, Shenyang, Peoples R China
[5] China Med Univ, Hosp 1, Dept Nucl Med, Shenyang, Peoples R China
关键词:
AJUBA;
NSCLC;
beta-Catenin;
P-ERK;
EPITHELIAL-MESENCHYMAL TRANSITION;
LIM PROTEIN AJUBA;
UP-REGULATION;
D O I:
10.1038/s41598-025-98156-z
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Accumulating evidence indicates that AJUBA acts as a potential target for new therapeutics to treat cancers. Nevertheless, the role of AJUBA in non-small cell lung cancer (NSCLC) remains unclear. In the current study, immunohistochemistry (IHC) showed that expression of AJUBA was upregulated in 67.55% of NSCLC tumor samples and was associated with tumor size, lymph node metastasis, advanced tumor stage, poor differentiation and poor prognosis. Loss-of-function assays of AJUBA produced by silencing RNA (siAJUBA) significantly inhibited the proliferation, invasion and migration of H1299 and A549 cell lines. Mechanistically, inhibition of extracellular signal-regulated kinases (ERKs) blocked the AJUBA-induced proliferation, invasion and migration of NSCLC cells, and decreased the expression of proteins related to the endothelial-mesenchymal transition (EMT). Silencing of AJUBA repressed tumor growth and led to a decrease in p-ERK, beta-catenin and N-cadherin in vivo. In conclusion,, overexpression of AJUBA facilitates the proliferation and motility of NSCLC cells via the ERK and Wnt/beta-catenin pathways. AJUBA may be useful as a prognostic marker which may provide a promising approach for the treatment of NSCLC.
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页数:12
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