Histochemical assessment of the femora of spontaneously diabetic torii-leprfa (SDT-fa/fa) rats that mimic type II diabetes

被引：0
作者：
Yao, Qi [1 ,2 ]
Tsuboi, Kanako [4 ]
Hongo, Hiromi [1 ]
Sakakibara, Mako [1 ,3 ]
Yamamoto, Tomomaya [1 ,6 ]
Haraguchi-Kitakamae, Mai [1 ]
Ishizu, Hotaka [5 ]
Liu, Xuanyu [1 ]
Shi, Yan [1 ]
Li, Weisong [1 ]
Cui, Jiaxin [1 ]
Shimizu, Tomohiro [5 ]
Amizuka, Norio [1 ]
Yokoyama, Atsuro [2 ]
Hasegawa, Tomoka [1 ]
Sakaguchi, Kiwamu [2 ]
机构：
[1] Hokkaido Univ, Fac Dent Med, Ultrastruct Hard Tissues, Sapporo, Japan
[2] Hokkaido Univ, Fac Dent Med, Oral Funct Prosthodont, Sapporo, Japan
[3] Hokkaido Univ, Fac Dent Med, Orthodont, Sapporo, Japan
[4] Haibara Gen Hosp, Dept Oral & Maxillofacial Surg, Makinohara, Japan
[5] Hokkaido Univ, Fac Med, Orthopaed Surg, Sapporo, Japan
[6] Japan Ground Selfdef Force Camp, Dept Dent, Shinmachi, Japan
来源：
JOURNAL OF ORAL BIOSCIENCES | 2025年 / 67卷 / 01期
基金：
日本学术振兴会;
关键词：
Diabetes; Osteoporosis; Osteocyte; Histochemistry; SDT-fa/fa rat; BONE TURNOVER; FRACTURE RISK; SCLEROSTIN; MELLITUS; COMPLICATIONS; OSTEOPOROSIS; PROTEINS; STRENGTH; CELLS;
D O I：
10.1016/j.job.2024.100602
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: To elucidate the mechanisms underlying diabetic osteoporosis, we conducted a comprehensive histological examination of the femora of Spontaneously Diabetic Torii-Leprfa (SDT-fa/fa) rats, an established model of obesity-related type 2 diabetes. Materials and methods: Femora from 12 30-week-old male SDT-fa/fa rats and age-matched Sprague-Dawley (SD) rats (controls) were used for detailed histochemical analyses, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, tissue nonspecific alkaline phosphatase (ALP), phosphoethanolamine/phosphocholine phosphatase 1 (PHOSPHO1), dentin matrix protein 1 (DMP-1), matrix extracellular phosphoglycoprotein (MEPE), sclerostin, osteocalcin staining, silver impregnation, von Kossa staining, and micro-computed tomography (CT). Results: Micro-CT and hematoxylin-eosin staining demonstrated significantly reduced trabecular bone volume in the femoral metaphyses of SDT-fa/fa rats. Although the number of TRAP-positive osteoclasts per bone surface remained comparable between both groups, SDT-fa/fa rats exhibited reduced areas of ALP-positive and PHOSPHO1-reactive mature osteoblasts/BS. Silver impregnation revealed a well-organized osteocytic lacunar-canalicular system in both groups. However, immunostaining identified aberrant DMP-1 and MEPE expression localized predominantly in the lacunae in SDT-fa/fa rats and in the lacunae and canaliculi of SD rats. Additionally, intense osteocalcin and sclerostin immunoreactivity was detected in osteocytes, along with a higher proportion of osteocalcin-positive osteocytes in SDT-fa/fa rats, distinguishing them from controls. Conclusion: SDT-fa/fa rats displayed a significant decline in osteoblastic function and distinctive distribution patterns of osteocyte-derived peptides, suggesting that this diabetic model may manifest alterations in osteoblastic activity and the osteocytic lacunar-canalicular network.
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页数：8
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