Research advances CRISPR gene editing technology generated models in the study of epithelial ovarian carcinoma

被引:0
作者
Li, Xiaosen [1 ,2 ]
Wang, Zhenpeng [1 ]
Man, Xiaxia [1 ]
Dai, Xiangpeng [3 ,4 ]
Zhou, Qi [2 ,3 ,5 ,6 ]
Zhang, Songling [1 ]
机构
[1] First Hosp Jilin Univ, Gynecol & Obstet Ctr, Dept Gynecol Oncol, Changchun, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing, Peoples R China
[3] Jilin Univ, Hosp 1, Key Lab Organ Regenerat & Transplantat, Minist Educ, Changchun, Peoples R China
[4] Jilin Univ, Hosp 1, Natl Local Joint Engn Lab Anim Models Human Dis, Changchun, Peoples R China
[5] Chinese Acad Sci, Inst Stem Cell & Regenerat Med, Beijing, Peoples R China
[6] Univ Chinese Acad Sci, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Mouse models; Tumorigenesis; Epithelial ovarian carcinoma; CRISPR genome editing technology; Genetically engineered mouse model (GEMM); Therapeutic strategy; ONE-STEP GENERATION; MOUSE MODELS; CANCER; MICE; ELECTROPORATION; EFFICIENCY; DNA;
D O I
10.1016/j.ygyno.2025.02.022
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Epithelial ovarian carcinoma (EOC), the most lethal gynecologic cancer, is often diagnosed at advanced stages, which urge us to explore the novel therapeutic strategies. Mouse models have played a crucial role in elucidating the molecular mechanisms for the development ovarian cancer and its therapeutic strategies. However, there are still various challenges in modeling the genetic drivers of ovarian cancer in animal models. Here, we provided an overview of the research advances for the molecular mechanisms underlying EOC development, therapeutic strategies, the CRISPR genome editing technology and its generated EOC models. The review also comprehensively discussed the advantages and obstacles of CRISPR in generating EOC mouse models and the promising therapeutic approach by correcting the oncogenes of EOC through in vivo delivery of gene-edited components. The development of more precise animal models, along with a deeper understanding of EOC molecular mechanisms, will dramatically benefit the investigation and treatment of EOC. (c) 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).
引用
收藏
页码:34 / 44
页数:11
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