GelMA Hydrogels Integrated With aptamer CH6-Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration

被引：0

作者：

Hong, Shebin ^{[1
]}

Cui, Ya ^{[1
]}

He, Dongming ^{[1
]}

Wu, Hao ^{[1
]}

Jiang, Weidong ^{[1
]}

Cao, Jian ^{[1
]}

Wang, Xudong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Res Inst Stomatol, Chinese Acad Med Sci,Sch Med,Coll Stomatol Natl Ct, Shanghai 200011, Peoples R China

来源：

MACROMOLECULAR BIOSCIENCE | 2025年

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

aptamer CH6; gelatin methacryloyl; hydrogel; osteoporotic bone regeneration; tetrahedral DNA nanostructure; DELIVERY; SCAFFOLDS; ORIGAMI; DEFECT;

D O I：

10.1002/mabi.202400471

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteoporotic bone regeneration is challenging due to impaired bone formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular regeneration owing to their ability to enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role in bone regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, a novel osteoblast-targeted CH6 aptamer-functionalized TDN (TDN-CH6) is aimed to develop for osteoporotic mandibular regeneration. This results demonstrated that TDN-CH6 exhibits superior osteoblast specificity and efficient recruitment to bone fracture sites. Furthermore, TDN-CH6 significantly enhances cellular activity and osteogenic differentiation compared to TDN alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating TDN and TDN-CH6 shows improved biological performance and are favorable for osteoporotic mandibular regeneration, suggesting that this platform represents a promising strategy for addressing complex bone defects.

引用

页数：13