Canine Adipose-Derived Mesenchymal Stromal Cells Reduce Cell Viability and Migration of Metastatic Canine Oral Melanoma Cell Lines In Vitro

被引:1
作者
Teng, Fwu Shing [1 ]
Lainetti, Patricia de Faria
Franzoni, Mayara Simao [1 ]
Leis Filho, Antonio Fernando [1 ]
Gomes, Cristina de Oliveira Massoco Salles [2 ]
Laufer-Amorim, Renee [3 ]
Amorim, Rogerio Martins
Fonseca-Alves, Carlos Eduardo [1 ,4 ,5 ]
机构
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, BR-18618681 Botucatu, Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Vet Pathol, BR-05508270 Sao Paulo, Brazil
[3] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, BR-18618681 Botucatu, Brazil
[4] Inst Vet Oncol IOVET, BR-05027020 Sao Paulo, Brazil
[5] Vet Precis Lab, BR-18610034 Botucatu, Brazil
基金
巴西圣保罗研究基金会;
关键词
dog; melanocyte; interleukins; peripheral mononuclear cells; stem cells; STEM-CELLS; MALIGNANT-MELANOMA; GENE-THERAPY; CANCER; INTERLEUKIN-2; EXPRESSION; GROWTH; IL-12; IMMUNOTHERAPY; REGRESSION;
D O I
10.3390/vetsci11120636
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Canine oral melanoma (COM) is a promising target for immunomodulatory therapies aimed at enhancing the immune system's antitumor response. Given that adipose-derived mesenchymal stem cells (Ad-MSCs) possess immunomodulatory properties through cytokine release, we hypothesized that co-culturing Ad-MSCs and canine peripheral blood mononuclear cells (PBMCs) could stimulate interleukin (IL) production against melanoma cell lines (MCCLs) and help identify therapeutic targets. This study evaluated IL-2, IL-8, and IL-12 expressions in co-culture with MCCL, Ad-MSCs, and PBMCs and assessed the relationship between gene expression, cell viability, and migration. Using four experimental groups in a Transwell insert system to separate cell types, we found that Ad-MSCs can reduce MCCL migration and viability, though the effect may vary depending on each cell line's susceptibility. Furthermore, Ad-MSCs modified IL expression profiles in co-cultured cells. Our findings suggest that Ad-MSCs could have therapeutic potential for COM by inhibiting cell migration and reducing viability. However, deeper insights into Ad-MSC interactions with the tumor microenvironment and melanoma-specific factors will be essential to optimize therapeutic efficacy.
引用
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页数:18
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