Enhanced sulfate pseudo-affinity chromatography using monolith-like particle architecture for purifying SARS-CoV-2

被引：0

作者：

Kadoi, Kenji ^{[1
]}

Toba, Junya ^{[1
]}

Uehara, Ayana ^{[1
]}

Isoda, Norikazu ^{[2
]}

Sakoda, Yoshihiro ^{[2
]}

Iwamoto, Eri ^{[1
]}

机构：

[1] JNC Corp, Yokohama R&D Ctr, 5-1 Ookawa,Kanazawa ku, Yokohama, Kanagawa 2368605, Japan

[2] Hokkaido Univ, Fac Vet Med, Lab Microbiol, Kita 18,Nishi 9,Kita Ku, Sapporo, Hokkaido 0600818, Japan

来源：

VACCINE | 2025年 / 53卷

关键词：

Monolith; SARS-CoV-2; Chromatography; Purification; pseudo-affinity; CELLUFINE SULFATE; INFLUENZA-A; VACCINE; VIRUSES; PURIFICATION;

D O I：

10.1016/j.vaccine.2025.126951

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Traditional virus chromatographic purification face limitations owing to the small pore sizes of conventional resins, which restrict efficient virus binding. The newly developed MLP1000 DexS, a cellulose monolith-like particle (MLP) with large continuous pores (radius of 1.5 mu m) and a sulfate pseudo-affinity ligand, facilitates virus access to intraparticle surfaces and significantly enhances binding capacity. In this study, we investigated the effectiveness of MLP1000 DexS for purifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from Vero cells. Using a 0.29 mL column volume, we evaluated this resin through bind-elute mode chromatography under two load volume conditions (4.5 mL and 21 mL). MLP1000 DexS exhibited superior performance under high-loading conditions, achieving a high elution recovery of 59 % for the virus compared with that of 11-17 % for the commercial resins Cellufine Sulfate and Capto DeVirS. Additionally, the dsDNA removal capacity of MLP1000 DexS was 3.0-5.3-fold higher than that of the other resins. These findings suggest that MLP1000 DexS is an effective purification material for the downstream processing of live-attenuated and inactivated coronavirus vaccine production.

引用

页数：6