Recognition of novel proteins encoded by an aquareovirus using mass spectrometry

被引：1

作者：

Yu, Fei ^{[1
]}

Song, Siyang ^{[1
]}

Xu, Jiehua ^{[1
]}

Hao, Kai ^{[1
]}

Wang, Yu ^{[1
]}

Zhao, Zhe ^{[1
]}

机构：

[1] Hohai Univ, Coll Oceanog, Jiangsu Prov Engn Res Ctr Marine Bioresources Sust, Nanjing, Peoples R China

来源：

VIROLOGY | 2025年 / 601卷

基金：

中国国家自然科学基金;

关键词：

dsRNA virus; Aquareovirus; Polycistronic mRNA; Protein-coding ORF; GRASS CARP REOVIRUS; S1; MESSENGER-RNA; NONCANONICAL TRANSLATION; GENUS AQUAREOVIRUS; IDENTIFICATION; EXPRESSION; VIRUS;

D O I：

10.1016/j.virol.2024.110281

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aquareovirus: a genus of within the family Spinareoviridae, order Reovirales, infects aquatic animals. Their genomes comprise 11 segments of double-stranded RNA, which function directly as mRNAs upon release into the cytoplasm of infected cells. Here, liquid chromatography-tandem mass spectrometry was employed to annotate small coding ORFs in the Aquareovirus-C genome. Its plus-strand RNA of segment 8 (S8) contains a novel protein-coding frame (NS15), and S5 seems to has an additional reading frame (NS18) with a putative non-AUG initiation codon. Among them, NS15 polypeptide has been proved by immunoblotting assay. Remarkably, the S4 and S11 minus-strand mRNAs may encode polypeptides, suggesting ambisense polarity of the two segmented RNAs. And the newly discovered NS12 ORF in 2019, from viral tricistronic S7 mRNA, was also confirmed by this mass-spectrometry data. Taken together, these identified new ORFs reveal the genome-coding complexity of Aquareovirus-C.

引用

页数：8

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