Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells

被引：0
作者：
Manikandan, R. [1 ]
Devi, K. [1 ]
Rajalingam, D. [2 ]
机构：
[1] Annamalai Univ, Dept Pharm, Chidambaram, Tamil Nadu, India
[2] Kamalakshi Pandurangan Coll Pharm, Dept Pharmaceut Chem, Tiruvannamalai, Tamil Nadu, India
来源：
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH | 2025年 / 59卷 / 01期
关键词：
Cell viability; MTT assay; Neurodegenerative diseases; Neuroprotective agent; Pyrazole derivatives; SH-SY5Y cells; ALZHEIMERS-DISEASE; MONOAMINE-OXIDASE; 1-N-SUBSTITUTED THIOCARBAMOYL-3-PHENYL-5-THIENYL-2-PYRAZOLINES; MITOCHONDRIAL DYSFUNCTION; INDUCED APOPTOSIS; B INHIBITORS; BETA; DEATH; DEGENERATION; ANTIOXIDANT;
D O I：
10.5530/ijper.20256349
中图分类号：
G40 [教育学];
学科分类号：
040101 ; 120403 ;
摘要：
Aim: To carry out Synthesis and Evaluation of novel 2, 5-substituted Pyrazolone for Alzheimer's Disease (AD) has become a serious public health concern as a result of people living longer than ever before. It is critically necessary to discover a way to halt and postpone the illness. The present study aims to determine if new synthetic analogue pyrazole derivatives may protect against toxicity caused by A(325-35 and its underlying mechanisms in neuroblastoma cells like SH-SY5Y. PyRx 0.9 software was used for molecular docking studies. The cells of SH-SY5Y were preincubated for 30 min with varying doses of the generated compounds (C1-C10) to induce neurotoxicity. They were then grown in A(325-35 (25 mol/L) for 48 hr. Cell viability was determined by MTT assay. Results: Compounds C5 and C8 exhibited a better binding score -8.4k/ cal compared to other analogues. Synthesized compounds C5 and C8 inhibited A(325-35-induced apoptosis in SH-SY5Y cells and protected neural cells from damage. Conclusion: The MTT assay confirmed that compounds C5 and C8 significantly reduced A(325-35-induced toxicity among human neuroblastoma SH-SY5Y cells, demonstrating its neuroprotective properties.
引用
收藏
页码：s323 / s332
页数：10
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