Protocol for evaluating drug-protein interactions based on fluorescence spectroscopy

被引：3

作者：

Jalali, Elaheh ^{[1
]}

Sargolzaei, Javad ^{[1
]}

机构：

[1] Arak Univ, Fac Sci, Dept Biol, Arak, Iran

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 04期

关键词：

ENANTIOMERS;

D O I：

10.1016/j.xpro.2024.103429

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigating the interactions between ligands, such as drugs, and proteins is essential to understand their functions and effects. This fluorescence spectroscopy protocol details how to analyze the effects of drugs on protein structure, including steps for solution preparation, fluorescence spectra collection, and results analysis. It is applicable to studying drug-protein interactions, binding affinity, and thermodynamic parameters as well as performing further studies on drug design.

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页数：10

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