Variation in innate immune responses to porcine epidemic diarrhea virus infection in piglets at different ages

被引：0

作者：

Lu, Yabin ^{[1
,2
]}

Li, Shuxian ^{[1
,2
]}

Yang, Shanshan ^{[2
]}

Wang, Caiying ^{[2
]}

Fu, Yuguang ^{[2
]}

Yu, Haoyuan ^{[2
]}

Huang, Xin ^{[2
]}

Zhao, Jing ^{[2
]}

Shao, Yongheng ^{[2
]}

Wang, Zemei ^{[2
]}

Cui, Yaru ^{[2
]}

Chen, Jianing ^{[2
]}

Guo, Qingyong ^{[1
]}

Kuang, Ling ^{[1
]}

Liu, Guangliang ^{[1
,2
]}

机构：

[1] Xinjiang Agr Univ, Coll Vet Med, Xinjiang Key Lab New Drug Study & Creat Herbivorou, Urumqi 830052, Peoples R China

[2] Lanzhou Univ, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Coll Vet Med, Lanzhou 730000, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2024年 / 196卷

基金：

中国国家自然科学基金;

关键词：

PEDV; Piglets; Age; Immune cells; Cytokines; ILEAL PEYERS-PATCHES; T-CELLS; EXPRESSION; SYSTEM;

D O I：

10.1016/j.micpath.2024.106958

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Porcine epidemic diarrhea virus (PEDV) poses a significant threat to pigs, with piglets under seven days old facing a mortality rate of up to 100 %. This study aimed to explore the maturation of the immune system in piglets across different age groups and their corresponding immune responses to PEDV infection. Real-time quantitative PCR was employed to assess the relative mRNA expression of inflammation-related factors in infected pigs compared to non-infected counterparts at varying ages. Additionally, flow cytometry was utilized to analyze the relative counts of CD4(+) and CD8(+) T cells, as well as CD21(+) B cells, in peripheral blood, spleen, mesenteric lymph nodes, and Peyer's patches of piglets at different developmental stages. Our findings revealed a notable increase in IFN-alpha and IFN-gamma, a decrease in TNF-alpha, and elevated expression of IL-1 beta, IL-6, IL-10, and IL-17 following PEDV infection. Furthermore, the numbers of CD4(+) and CD8(+) T cells, along with CD21(+) B cells, exhibited a gradual rise with the advancement of piglets' age. Overall, our study underscores the progressive enhancement of piglets' resistance to PEDV infection as their immune system matures over time.

引用

页数：10

共 37 条

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