Evaluation of the Sedative Effect of Limonene to Reduce Stress During Transportation of Gilthead Seabream (Sparus aurata)

被引：0
作者：
Simo-Mirabet, Paula [1 ]
Caderno, Anyell [1 ]
Flores-Llano, Maria Jose [1 ]
da Silva, Elisia Gomes [2 ]
Schoenau, William [2 ]
Baldisserotto, Bernardo [2 ]
Martinez-Rodriguez, Gonzalo [3 ]
Mancera, Juan Miguel [1 ]
Martos-Sitcha, Juan Antonio [1 ]
机构：
[1] Univ Cadiz, Inst Univ Invest Marina INMAR, Fac Marine & Environm Sci, Dept Biol, Campus Excelencia Int Mar CEI MAR, Puerto Real 11519, Spain
[2] Univ Fed Santa Maria, Dept Physiol & Pharmacol, BR-97105900 Santa Maria, RS, Brazil
[3] CSIC, Inst Marine Sci Andalusia ICMAN, Dept Marine Biol & Aquaculture, Puerto Real 11519, Spain
来源：
BIOLOGY-BASEL | 2025年 / 14卷 / 02期
关键词：
aquaculture procedures; essential oils; fish transportation; HPI axis; welfare; ESSENTIAL OIL; ALOYSIA-TRIPHYLLA; PHYSIOLOGICAL-RESPONSES; SODIUM-CHLORIDE; PLASMA-CORTISOL; SILVER CATFISH; R(+) LIMONENE; CITRUS-JUNOS; WATER; FISH;
D O I：
10.3390/biology14020115
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Reducing stress during common aquaculture practices such as transportation is essential for improving animal welfare and aquaculture productivity. This study assessed the sedative effect of limonene, a key component of citrus essential oils, in a 6 h transport simulation of gilthead seabream (Sparus aurata), including four experimental groups: (i) non-transported, (ii) control (without treatment), (iii) ethanol (with ethanol), and (iv) limonene (30 mu L/L dissolved in ethanol). After transport, the slightly reduced O2 consumption observed in the limonene group could be indicative of a reduced metabolism supported by a significant increase of chloride levels concerning other transported groups and an increase of sodium ions in fish treated with limonene. The increase of cortisol in transported groups supports its use as a good marker of acute-stress response in fish transport, showing a smaller increase in fish treated with limonene. This fact, together with the significantly reduced glucose and increased triglycerides in plasma, and the reduced expression of stress-related genes in the head kidney, may suggest a more relaxed state in fish treated with limonene. Since the sedative benefits of limonene were partly masked by the negative effects of ethanol, finding an alternative solvent could enhance its potential as a sedative for fish transport.
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页数：20
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  • [1] Ashley P.J., Fish welfare: Current issues in aquaculture, Appl. Anim. Behav. Sci, 104, pp. 199-235, (2007)
  • [2] Schreck C.B., Tort L., The Concept of Stress in Fish, Fish Physiology: Biology of Stress in Fish, pp. 1-34, (2016)
  • [3] Aydin B., Barbas L.A.L., Sedative and anesthetic properties of essential oils and their active compounds in fish: A review, Aquaculture, 520, (2020)
  • [4] Nair V.R., Parvathy U., Jithin T.J., Binsi P.K., Ravishankar C.N., Live transportation of food fishes: Current scenario and future prospects, Curr. Sci, 124, (2023)
  • [5] Fang D., Mei J., Xie J., Qiu W., The Effects of Transport Stress (Temperature and Vibration) on Blood Biochemical Parameters, Oxidative Stress, and Gill Histomorphology of Pearl Gentian Groupers, Fishes, 8, (2023)
  • [6] Martin I., Gutierrez J.R., Martos-Sitcha J.A., Rasines I., Rodriguez C., Mancera J.M., Chereguini O., Prolonged emersion of Solea senegalensis, Kaup 1858, for its application in transport, Aquac. Res, 48, pp. 3393-3400, (2017)
  • [7] Bonga S.W., Hormonal responses to stress|Hormone Response to Stress, Encyclopedia of Fish Physiology, pp. 1515-1523, (2011)
  • [8] Gorissen M., Flik G., The Endocrinology of the Stress Response in Fish, Fish Physiology: Biology of Stress in Fish, pp. 75-111, (2016)
  • [9] Souza C.F., Baldissera M.D., Baldisserotto B., Heinzmann B.M., Martos-Sitcha J.A., Mancera J.M., Essential oils as stress-reducing agents for fish aquaculture: A review, Front. Physiol, 10, (2019)
  • [10] Ross L.G., Ross B., Anaesthetic and Sedative Techniques for Aquatic Animals, (2008)
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