Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

被引：0

作者：

Krell, Johannes ^{[1
]}

Javarappa, Komal Kumar ^{[2
]}

Wenedy, Angie ^{[1
,2
]}

Frelinger, Andrew L., III

Renia, Laurent ^{[3
,4
]}

Costa, Clarissa Prazeres da ^{[5
,6
,7
]}

Schlegel, Martin ^{[1
]}

Knolle, Percy ^{[8
]}

Schneider, Gerhard ^{[1
]}

Hayden, Oliver ^{[9
,10
]}

机构：

[1] TUM Univ Hosp Munchen, Dept Anesthesiol & Intens Care Med, Munich, Germany

[2] TUM Create Ltd, CREATE Way 10-02,CREATE Tower, Singapore 138602, Singapore

[3] ASTAR, ASTAR Infect Dis Labs, Singapore, Singapore

[4] Nanyang Technol Univ, Sch CE, Singapore, Singapore

[5] German Ctr Infect Res DZIF, Partner Site Munich, Munich, Germany

[6] TUM, Ctr Global Hlth, Sch Med & Hlth, Munich, Germany

[7] TUM, Inst Med Mikrobiol Immunol & Hyg, Sch Med & Hlth, Trogerstr 30, D-81675 Munich, Germany

[8] Tech Univ Munich, Klinikum Rechts Isar, Inst Mol Immunol & Expt Oncol, D-81675 Munich, Germany

[9] TUM, Heinz Nixdorf Chair Biomed Elect, TranslaTUM, Sch Computat Informat & Technol, Einsteinstr 25, D-81675 Munich, Germany

[10] TUM, Munich Inst Biomed Engn, D-85748 Munich, Germany

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

Clinical Protocol; Flow Cytometry; Immunology; Microscopy;

D O I：

10.1016/j.xpro.2025.103598

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.

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页数：21

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