Determination of the Infection Dynamics of Escherichia coli O157:H7 by Bacteriophage ΦV10

被引：0
作者：
Oats, Michael F. [1 ]
Coronel-Aguilera, Claudia P. [2 ]
Applegate, Bruce M. [1 ,2 ,3 ]
Csonka, Laszlo N. [1 ]
Bhunia, Arun K. [2 ,3 ,4 ]
Gehring, Andrew G. [5 ]
Paoli, George C. [5 ]
机构：
[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA
[3] Purdue Univ, Interdisciplinary Life Sci Program PULSe, W Lafayette, IN 47907 USA
[4] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA
[5] US Dept Agr, Eastern Reg Res Ctr, Agr Res Serv, Characterizat & Intervent Foodborne Pathogens Res, Wyndmoor, PA 19038 USA
来源：
FOODS | 2025年 / 14卷 / 04期
基金：
美国食品与农业研究所;
关键词：
bacteriophage; <italic>E. coli</italic> O157:H7; Phi V10; phage binding; SENSITIVE DETECTION;
D O I：
10.3390/foods14040617
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Phi V10 is an Escherichia coli O157:H7-specific bacteriophage that has been used to develop luminescent reporter assays for the detection of this important foodborne pathogen. Previous work demonstrated the specificity of Phi V10 for infection of E.coli O157:H7 through interaction with the O157 antigen. In addition, modification of the lipopolysaccharide (LPS) via O-acetylation prevents Phi V10 infection in an E. coli O157:H7 expressing a phage-encoded O-acetylase gene. Through assays for phage binding, plaque formation, and lysogeny using non-O157:H7 and O157: non-H7 strains, as well as complementation of an O157:H- strain, it is demonstrated in this study that both the somatic O157 antigen and flagellar H7 antigen are required for productive infection of E. coli O157:H7 by Phi V10. Together, the results indicate that the O157 antigen is required for phage binding and that the H7 antigen is necessary to complete the infection process.
引用
收藏
页数：12
相关论文
共 31 条
  • [1] Ameer M.A., Wasey A., Salen P., Escherichia coli (E. coli O157 H7), StatPearls, (2023)
  • [2] Croxen M.A., Law R.J., Scholz R., Keeney K.M., Wlodarska M., Finlay B.B., Recent advances in understanding enteric pathogenic Escherichia Coli, Clin. Microbiol. Rev, 26, pp. 822-880, (2013)
  • [3] Castro V.S., Carvalho R.C.T., Conte-Junior C.A., Figuiredo E.E.S., Shiga-toxin producing Escherichia coli: Pathogenicity, supershedding, diagnostic methods, occurrence, and foodborne outbreaks, Compr. Rev. Food Sci. Food Saf, 16, pp. 1269-1280, (2017)
  • [4] Spalding A., Goodhue R.E., Kiesel K., Sexton R.J., Economic damages of food-safety incidents in complex markets: 2018 E. coli outbreak and romaine lettuce industry, Am. J. Agric. Econ, 105, pp. 597-623, (2023)
  • [5] Batz M.B., Hoffmann S., Morris J.G., Ranking the disease burden of 14 pathogens in food Sources in the United States using attribution data from outbreak investigations and expert elicitation, J. Food Prot, 75, pp. 1278-1291, (2012)
  • [6] Kabiraz M.P., Majumdar P.R., Mahmud M.M.C., Bhowmik S., Ali A., Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review, Heliyon, 9, (2023)
  • [7] He L., Yang H., Xiao P., Singh R., He N., Liu B., Li Z., Highly selective, sensitive and rapid detection of Escherichia coli O157:H7 using duplex PCR and magnetic nanoparticle-based chemiluminescence assay, J. Biomed. Nanotechnol, 13, pp. 1243-1252, (2017)
  • [8] Wang Z., Yao X., Zhang Y., Wang R., Ji Y., Sun J., Zhang D., Wang J., Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7, Food Chem, 329, (2020)
  • [9] Zhao Y., Zeng D., Yan C., Chen W., Ren J., Jiang Y., Jiang L., Xue F., Ji D., Tang F., Et al., Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA, Analyst, 145, pp. 3106-3115, (2020)
  • [10] Hu X., Li Y., Xu Y., Gan Z., Zou X., Shi J., Huang X., Li Z., Li Y., Green one-step synthesis of carbon quantum dots from orange peel for fluorescent detection of Escherichia coli in milk, Food Chem, 339, (2021)
1234