COVID-19 Induces Greater NLRP3 Inflammasome Activation in Obese Patients than Other Chronic Illnesses: A Case-Control Study

被引：0

作者：

D'Amico, Raissa Campos ^{[1
,2
]}

Nagashima, Seigo ^{[1
]}

Carstens, Lucas Baena ^{[1
,2
]}

Bertoldi, Karina de Guadalupe ^{[1
]}

Mataruco, Sabrina ^{[2
]}

Honorio D'Agostini, Julio Cesar ^{[3
]}

Hlatchuk, Elisa Carolina ^{[3
]}

da Silva, Sofia Brunoro ^{[2
]}

de Noronha, Lucia ^{[1
,2
,3
]}

Baena, Cristina Pellegrino ^{[1
,2
]}

机构：

[1] Pontificia Univ Catolica Parana, Postgrad Program Hlth Sci PPGCS, BR-80215901 Curitiba, PR, Brazil

[2] Pontificia Univ Catolica Parana, Sch Med, BR-80215901 Curitiba, PR, Brazil

[3] Univ Fed Parana, Sch Med, BR-80060240 Curitiba, PR, Brazil

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2025年 / 26卷 / 04期

关键词：

COVID-19; SARS-CoV-2; inflammasome; pyroptosis; obesity; HYPOXIA; INTERLEUKIN-6; CASPASES; ROLES;

D O I：

10.3390/ijms26041541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Obesity has been identified as an independent risk factor for severe COVID-19 unfavorable outcomes. Several factors, such as increased ACE2 receptor expression and chronic inflammation, can contribute to this relationship, yet the activation of the NLRP3 inflammasome pathway is also a key element. Our primary goal was to determine whether chronic NLRP3 inflammasome activation in people with obesity is different in critical COVID-19 and in critical chronic conditions. A retrospective analysis was conducted using clinical data and post-mortem lung tissue samples from 14 COVID-19 patients with obesity (group A) and 9 patients with obesity who died from non-COVID-19 causes (group B). Immunohistochemical analysis assessed twelve markers related to the NLRP3 inflammasome pathway. Group A showed a significantly higher expression of ASC (p = 0.0387) and CASP-1 (p = 0.0142). No significant differences were found for IL-8, TNF-alpha, NF-kB, NLRP3, IL-1 beta, and gasdermin-D. Group B had higher levels of IL-6 (p < 0.0001), IL-18 (p = 0.002), CASP-9 (p < 0.0001), and HIF (p = 0.0327). We concluded that COVID-19 activates the NLRP3 inflammasome pathway, possibly leading to pyroptotic cell death mediated by caspase-1. In contrast, people with obesity without COVID-19, despite exhibiting some markers of the NLRP3 inflammasome, are more likely to experience necroptosis mediated by caspase-9.

引用

页数：14

共 69 条

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