Diversity and Pathogenicity of Colletotrichum Species Causing Coffee Anthracnose in China

被引：0
作者：
Lu, Ying [1 ,2 ]
Zhang, Weiyi [3 ]
Hu, Xiaoli [4 ]
He, Chunping [1 ,2 ]
Liang, Yanqiong [1 ,2 ]
Huang, Xing [1 ,2 ]
Yi, Kexian [1 ,2 ,5 ]
Wu, Weihuai [1 ,2 ]
机构：
[1] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Peoples R China
[2] Hainan Key Lab Monitoring & Control Trop Agr Pests, Haikou 571101, Peoples R China
[3] Hainan Univ, Sch Trop Agr & Forestry, Danzhou 571737, Peoples R China
[4] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
[5] Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Peoples R China
来源：
MICROORGANISMS | 2025年 / 13卷 / 03期
基金：
国家重点研发计划;
关键词：
coffee anthracnose; Colletotrichum spp. pathogenicity tests; diagnosis; taxonomy; MOLECULAR CHARACTERIZATION; DISEASE; NYMPHAEAE; KERATITIS; BERRIES; COMPLEX;
D O I：
10.3390/microorganisms13030512
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Coffee is a significant traded commodity for developing countries. Among the various diseases affecting coffee, anthracnose caused by Colletotrichum spp. has re-emerged as a major constraint on global coffee production. To better understand the Colletotrichum species complex associated with coffee anthracnose, we characterized Colletotrichum spp. using a combination of phenotypic traits, MAT1-2 (ApMat) gene analysis, multi-locus phylogenetic (ITS, ACT, CHS-1, and GAPDH), and pathogenicity assays. A total of 74 Colletotrichum isolates were collected from coffee plants exhibiting anthracnose symptoms across nine coffee plantations in China. Among these, 55 isolates were identified as the C. gloeosporioides species complex using the ApMat locus, while the remaining 19 isolates were identified through multi-locus phylogenetic analyses. The isolates represented seven Colletotrichum species from five species complexes: C. gloeosporioides (including C. siamense, C. nupharicola, and C. theobromicola), C. boninens (C. karstii), C. gigasporum (C. gigasporum), C. orchidearum (C. cliviicola), and C. magnum (C. brevisporum). This is the first report of C. nupharicola and C. cliviicola causing coffee anthracnose worldwide, and the first report of C. nupharicola in China. Pathogenicity tests confirmed that all seven species were capable of infecting coffee leaves. This research enhances our understanding of the Colletotrichum species responsible for coffee anthracnose, and provides valuable insights for developing effective disease management strategies.
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页数：19
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