β-Sitosterol Improves Synaptic Plasticity in High-risk Children with Cerebral Palsy by Regulating Inflammatory Homeostasis

被引:0
作者
Luan, Shaoyong [1 ]
Wang, Peipei [1 ]
Wang, Caixia [1 ]
机构
[1] Qingdao Municipal Hosp, Dept Pediat NICU, Qingdao, Shandong, Peoples R China
关键词
beta-Sitosterol; nerve growth factor; inflammatory homeostasis; cerebral palsy; synaptic plasticity; NERVE GROWTH-FACTOR; NGF;
D O I
10.1177/09731296251324723
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Background Cerebral palsy (CP) is a serious childhood disease characterized by changes in inflammatory responses and synaptic plasticity. Recent evidence highlighted the anti-inflammatory properties of beta-sitosterol and that the nerve growth factor (NGF) regulates neuronal and synaptic plasticity.Purpose This study aimed to explore the mechanism by which beta-sitosterol regulates inflammatory homeostasis through NGF, thereby improving synaptic plasticity in high-risk children with CP.Methods A rat model of CP was constructed, and the animals were divided into a model group, beta-sitosterol group, beta-sitosterol group + NGF group, and beta-sitosterol + K252 alpha group. Following treatment, the Morris water maze and Bederson score were used to evaluate the behavioral performance of rats, Western blot analysis was used to detect the expression of NGF and hippocampal protein, and enzyme-linked immunosorbent assay (ELISA) was used to measure inflammatory factor levels. Additionally, immunofluorescence examined the expression of synaptophysin in rat brains, and the brain tissue was observed by hematoxylin and eosin (HE) staining.Results The CP rat model was successfully constructed. Of note, beta-sitosterol treatment improved the synaptic plasticity of the CP rats with decreased Abnormal Involuntary Movement Scale (AIMS) and Bederson scores and a shorter latent period. Moreover, beta-sitosterol inhibited the production of pro-inflammatory factors and increased the number of synapses in the hippocampus of rats while increasing the expression of brain-derived neurotrophic factor (BDNF), SYN, N-methyl d-aspartate receptor subtype 2B (NR2B), and NGF. Interestingly, administration of NGF inhibitor enhanced the inflammation response and decreased the protein expressions in synaptic receptors.Conclusion beta-Sitosterol improves synaptic plasticity in high-risk children with CP and alleviates inflammation and nerve cell apoptosis through up-regulation of NGF expression.
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页数:11
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