Proline/serine-rich coiled-coil protein 1 alleviates pyroptosis in murine bone marrow-derived macrophages

被引：0

作者：

Wu, Qiao ^{[1
]}

Wang, Qianqian ^{[1
]}

Hu, Kexin ^{[1
]}

Luo, Tiantian ^{[2
]}

Liu, Jichen ^{[3
]}

Xue, Yazhi ^{[4
]}

Li, Ling ^{[1
]}

Yang, Cuiqi ^{[1
]}

Lin, Rongzhan ^{[1
,3
]}

Pan, Hangyu ^{[3
]}

Wang, Jinhao ^{[1
]}

Guo, Zhigang ^{[1
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Huiqiao Med Ctr, Dept Cardiol, Guangzhou 510080, Peoples R China

[2] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Dept Cardiol, Chengdu 610014, Peoples R China

[3] Southern Med Univ, Nanfang Hosp, Dept Cardiol, State Key Lab Organ Failure Res, Guangzhou 510080, Peoples R China

[4] Southern Med Univ, Nanfang Hosp, Dept Gen Practice, Guangzhou 510080, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2025年 / 57卷 / 09期

基金：

中国国家自然科学基金;

关键词：

proline/serine-rich coiled-coil protein 1; pyroptosis; bone marrow-derived macrophages; inflammation; SPINDLE; DDA3;

D O I：

10.3724/abbs.2025012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyroptosis is a regulated inflammatory cell death process that plays an essential role in various diseases. This study investigates the role of proline/serine-rich coiled-coil protein 1 (PSRC1) in pyroptosis and inflammation in macrophages. This study reports that PSRC1 expression is decreased in pyroptotic macrophages and that knockout of PSRC1 exacerbates pyroptosis and inflammation. PSRC1 overexpression alleviates pyroptosis and inflammation in macrophages. RNA-seq analysis reveals that PSRC1 regulates the expression of genes involved in the extracellular matrix (ECM). Specifically, PSRC1 downregulates the expression of periostin (POSTN), an ECM component. Knockdown of POSTN suppresses macrophage pyroptosis mediated by low expression of PSRC1. These findings suggest that PSRC1 can alleviate pyroptosis and inflammation in bone marrow-derived macrophages (BMDMs) by regulating the ECM and negatively regulating POSTN. This study provides insights into the role of PSRC1 in macrophage pyroptosis and identifies a potential target for the treatment of inflammatory diseases. Further research is needed to confirm these findings in vivo and in various disease models.

引用

页数：13

共 37 条

[31] Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration [J].

Wu, Jinna ;

Chen, Yuyu ;

Liao, Zhiheng ;

Liu, Hengyu ;

Zhang, Shun ;

Zhong, Dongmei ;

Qiu, Xianjian ;

Chen, Taiqiu ;

Su, Deying ;

Ke, Xiaona ;

Wan, Yong ;

Zhou, Taifeng ;

Su, Peiqiang .

MOLECULAR THERAPY, 2022, 30 (10) :3241-3256

[32] Integrated Analysis Reveals the Gut Microbial Metabolite TMAO Promotes Inflammatory Hepatocellular Carcinoma by Upregulating POSTN [J].

Wu, Yonglin ;

Rong, Xingyu ;

Pan, Miaomiao ;

Wang, Tongyao ;

Yang, Hao ;

Chen, Xiejiu ;

Xiao, Zhenming ;

Zhao, Chao .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2022, 10

[33] Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling [J].

Xian, Hongxu ;

Watari, Kosuke ;

Sanchez-Lopez, Elsa ;

Offenberger, Joseph ;

Onyuru, Janset ;

Sampath, Harini ;

Ying, Wei ;

Hoffman, Hal M. ;

Shadel, Gerald S. ;

Karin, Michael .

IMMUNITY, 2022, 55 (08) :1370-+

[34] HMGB1 as a therapeutic target in disease [J].

Xue, Jiaming ;

Suarez, Joelle S. ;

Minaai, Michael ;

Li, Shuangjing ;

Gaudino, Giovanni ;

Pass, Harvey I. ;

Carbone, Michele ;

Yang, Haining .

JOURNAL OF CELLULAR PHYSIOLOGY, 2021, 236 (05) :3406-3419

[35] N-Cadherin Overexpression Mobilizes the Protective Effects of Mesenchymal Stromal Cells Against Ischemic Heart Injury Through a β-Catenin-Dependent Manner [J].

Yan, Wenjun ;

Lin, Chen ;

Guo, Yongzhen ;

Chen, Youhu ;

Du, Yunhui ;

Lau, Wayne Bond ;

Xia, Yunlong ;

Zhang, Fuyang ;

Su, Renzhi ;

Gao, Erhe ;

Wang, Yajing ;

Li, Congye ;

Liu, Rui ;

Ma, Xin-liang ;

Tao, Ling .

CIRCULATION RESEARCH, 2020, 126 (07) :857-874

[36] Periostin aggravates NLRP3 inflammasome-mediated pyroptosis in myocardial ischemia-reperfusion injury [J].

Yao, Lei ;

Song, Jie ;

Meng, Xiao Wen ;

Ge, Jian Yun ;

Du, Bo Xiang ;

Yu, Jun ;

Ji, Fu Hai .

MOLECULAR AND CELLULAR PROBES, 2020, 53

[37] Pyroptosis in cardiovascular diseases: Pumping gasdermin on the fire [J].

Yarovinsky, Timur O. ;

Su, Meiling ;

Chen, Chaofei ;

Xiang, Yaozu ;

Tang, Wai Ho ;

Hwa, John .

SEMINARS IN IMMUNOLOGY, 2023, 69

← 1 2 3 4 →