Succinic acid synthesis regulated by succinyl-coenzyme A ligase (SUCLA) plays an important role in root response to alkaline salt stress in Leymus chinensis

被引：2

作者：

Wu, Jing ^{[1
]}

Sun, Mengtong ^{[1
]}

Pang, Anqi ^{[1
]}

Ma, Kaiman ^{[1
]}

Hu, Xuefei ^{[1
]}

Feng, Shuang ^{[2
]}

Wang, Yue ^{[1
]}

Zhou, Aimin ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China

[2] Northeast Agr Univ, Large Scale Instrument & Equipment Sharing Serv Pl, Harbin 150030, Peoples R China

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2025年 / 220卷

基金：

中国国家自然科学基金;

关键词：

Alkaline salt; Leymus chinensis; Succinic acid; Succinyl-coenzyme A ligase (SUCLA); COA SYNTHETASE; ALPHA;

D O I：

10.1016/j.plaphy.2025.109485

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Alkaline salts have more severe adverse effects on plant growth and development than neutral salts do. However, the adaptive mechanisms of plants to alkaline salt stress remain poorly understood, especially at the molecular level. The Songnen Plain in northeast China is composed of typical 'soda' saline-alkali soil, with NaHCO3 and Na2CO3 as the predominant alkaline salts (pH >= 9.2). Leymus chinensis can grow on this saline-alkali land, showing strong adaptability. We investigated the role of succinic acid and genes regulating its synthesis in the response to alkaline salt stress in L. chinensis roots. Compared to the neutral salt (NaCl) and high pH treatments, the alkaline salt (NaHCO3 and Na2CO3) treatment specifically caused changes in 11 organic acids, of which the increase in succinic acid was the greatest. The exogenous addition of succinic acid alleviates the damage of alkaline salt to L. chinensis roots. Further, two genes encoding succinyl-coenzyme A ligase (SUCLA) subunits that regulate succinic acid synthesis, LcSUCLA alpha and LcSUCLA(3, were identified; these genes interact and were localized within mitochondria. Overexpression of LcSUCLA alpha and LcSUCLA beta caused an increase in succinic acid and enhanced tolerance of NaHCO3 in transgenic rice seedlings. These results suggest that LcSUCLA alpha and LcSUCLA(3 may be involved in the response to alkaline salt stress through the regulation of succinic acid synthesis.

引用

页数：10

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