Pasteurella multocida Serotype D Infection Induces Activation of the IL-17 Signaling Pathway in Goat Lymphocytes

被引：0

作者：

Fu, Yujing ^{[1
]}

Meng, Yong ^{[1
]}

Qian, Hejie ^{[1
]}

Chen, Taoyu ^{[1
]}

Chen, Xiangying ^{[1
]}

Chen, Qiaoling ^{[1
]}

Gao, Hongyan ^{[1
]}

Man, Churiga ^{[1
]}

Du, Li ^{[1
]}

Chen, Si ^{[1
]}

Wang, Fengyang ^{[1
]}

机构：

[1] Hainan Univ, Coll Trop Agr & Forestry, Anim Genet Engn Key Lab Haikou, Hainan Key Lab Trop Anim Reprod & Breeding & Epide, Haikou 570228, Peoples R China

来源：

MICROORGANISMS | 2024年 / 12卷 / 12期

基金：

海南省自然科学基金;

关键词：

<italic>Pasteurella multocida</italic> type D; peripheral blood lymphocytes; IL-17 signaling pathway; cytokines; APOPTOSIS; LIPOPOLYSACCHARIDE; INDUCTION; SEPSIS; CELLS;

D O I：

10.3390/microorganisms12122618

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

(1) Background: Pasteurellosis is a global zoonotic bacterial disease, which has caused significant economic impacts in animal husbandry. Nevertheless, there is limited understanding of the immune response between goat peripheral blood lymphocytes (PBLs) and goat-derived Pasteurella multocida (P. multocida). (2) Methods: To investigate the immune response of host PBLs during infection with P. multocida type D, we established an in vitro cell model utilizing isolated primary goat PBLs. Utilizing this in vitro infection model, we employed an enzyme-linked immunosorbent assay (ELISA) to assess the cytokine profile variation in goat PBLs following infection. Meanwhile, RNA sequencing and quantitative PCR (qPCR) methods were employed to analyze the gene expression profile. (3) Results: The ELISA test results indicated that the expression levels of pro-inflammatory cytokines, such as IL-6, IFN-gamma, CXCL10, and IL-17A, were significantly elevated within 12 h after infection with P. multocida. In contrast, the levels of the anti-inflammatory cytokine IL-10 were found to be reduced. RNA sequencing and functional enrichment analysis identified 2114 differentially expressed genes (DEGs) that were primarily associated with cytokine-cytokine receptor interactions, viral protein-cytokine interactions, and the IL-17 signaling pathway. Furthermore, protein-protein interaction (PPI) network analysis and qPCR highlighted CD86, CCL5, CD8A, CXCL8, CTLA4, TNF, CD274, IL-10, IL-6, CXCL10, IFNG, and IL-17A that were crucial for the response of PBLs to P. multocida infection. (4) Conclusions: This study systematically revealed the characteristics of PBLs in goats following infection with goat-derived P. multocida type D through the analysis of cytokines and gene expression, providing important theoretical insights for a deeper understanding of the defense mechanisms in goats against P. multocida.

引用

页数：15

共 45 条

[1] Wilson B.A., Ho M., Pasteurella multocida: From zoonosis to cellular microbiology, Clin. Microbiol. Rev, 26, pp. 631-655, (2013)
[2] Chen Q., Zhang Z., Chen S., Chen J., Cheng Y., Liu A., Li B., Chen Z., Zheng Y., Ga M., Et al., Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated with Immune Reaction to Pasteurella multocida in Goats, Front. Vet. Sci, 8, (2021)
[3] Peng Z., Wang X., Zhou R., Chen H., Wilson B.A., Wu B., Pasteurella multocida: Genotypes and Genomics, Microbiol. Mol. Biol. Rev. MMBR, 83, (2019)
[4] Almoheer R., Abd Wahid M.E., Zakaria H.A., Jonet M.A.B., Al-Shaibani M.M., Al-Gheethi A., Addis S.N.K., Spatial, Temporal, and Demographic Patterns in the Prevalence of Hemorrhagic Septicemia in 41 Countries in 2005–2019: A Systematic Analysis with Special Focus on the Potential Development of a New-Generation Vaccine, Vaccines, 10, (2022)
[5] Watson P.J., Davies R.L., Outbreak of Pasteurella multocida septicaemia in neonatal lambs, Vet. Rec, 151, pp. 420-422, (2002)
[6] Rahman M.H., Akther S., Alam M.S., Hassan M.Z., Sarker M.S., Ali M.Z., Giasuddin M., Ahmed S., Prevalence and identification of caprine pasteurellosis in pneumonic goats in Bangladesh, J. Adv. Vet. Anim. Res, 10, pp. 538-544, (2023)
[7] Guan L., Xue Y., Ding W., Zhao Z., Biosynthesis and regulation mechanisms of the Pasteurella multocida capsule, Res. Vet. Sci, 127, pp. 82-90, (2019)
[8] Peng Z., Liang W., Wang F., Xu Z., Xie Z., Lian Z., Hua L., Zhou R., Chen H., Wu B., Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates from Different Host Species, Front. Microbiol, 9, (2018)
[9] Gharib Mombeni E., Gharibi D., Ghorbanpoor M., Jabbari A.R., Cid D., Toxigenic and non-toxigenic Pasteurella multocida genotypes, based on capsular, LPS, and virulence profile typing, associated with pneumonic pasteurellosis in Iran, Vet. Microbiol, 257, (2021)
[10] An Q., Chen S., Zhang L., Zhang Z., Cheng Y., Wu H., Liu A., Chen Z., Li B., Chen J., Et al., The mRNA and miRNA profiles of goat bronchial epithelial cells stimulated by Pasteurella multocida strains of serotype A and D, PeerJ, 10, (2022)

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