Selection of Sclerodermus pupariae Reference Genes for Quantitative Real-Time PCR

被引：0
|
作者：
Zhou, Ting [1 ]
Feng, Huahua [1 ]
Zhang, Jie [2 ]
Tang, Yanlong [2 ]
Dong, Xiaoling [1 ]
Kang, Kui [2 ,3 ]
机构：
[1] Yangtze Univ, Coll Agr, MARA Key Lab Sustainable Crop Prod Middle Reaches, Jingzhou 434025, Peoples R China
[2] Zunyi Normal Univ, Coll Biol & Agr, Zunyi 563006, Peoples R China
[3] Hubei Univ Med, Biomed Res Inst, Shiyan 442000, Peoples R China
来源：
INSECTS | 2025年 / 16卷 / 03期
基金：
中国国家自然科学基金;
关键词：
S; pupariae; reference gene screening; qRT-PCR; developmental stages; EXPRESSION; NORMALIZATION;
D O I：
10.3390/insects16030268
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
S. pupariae is a newly discovered species of parasitoid wasps. Research into its development, behavioral genetics, and molecular mechanisms provides valuable insights for improving integrated pest management strategies. Quantitative real-time PCR (qRT-PCR) is the most commonly used method for analyzing gene expression. This method requires the identification of stable reference genes to accurately evaluate transcriptional level variations. In this study, eight candidate reference genes (TUB, TBP, RPS18, GAPDH, 18S rRNA, RPL32, Actin, and EF1-alpha) were identified and evaluated for their suitability as reference genes. Gene expression levels across different developmental stages were analyzed using three software tools, GeNorm, NormFinder, and BestKeeper, and the online tool RefFinder. The overall ranking of reference gene stability was as follows: RPS18 > 18S rRNA > RPL32 > GAPDH > Actin > TUB > TPB > EF1-alpha. Ultimately, RPS18 was determined to be the most stable reference gene.
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页数：12
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