Selection of Sclerodermus pupariae Reference Genes for Quantitative Real-Time PCR

被引：0
|
作者：
Zhou, Ting [1 ]
Feng, Huahua [1 ]
Zhang, Jie [2 ]
Tang, Yanlong [2 ]
Dong, Xiaoling [1 ]
Kang, Kui [2 ,3 ]
机构：
[1] Yangtze Univ, Coll Agr, MARA Key Lab Sustainable Crop Prod Middle Reaches, Jingzhou 434025, Peoples R China
[2] Zunyi Normal Univ, Coll Biol & Agr, Zunyi 563006, Peoples R China
[3] Hubei Univ Med, Biomed Res Inst, Shiyan 442000, Peoples R China
来源：
INSECTS | 2025年 / 16卷 / 03期
基金：
中国国家自然科学基金;
关键词：
S; pupariae; reference gene screening; qRT-PCR; developmental stages; EXPRESSION; NORMALIZATION;
D O I：
10.3390/insects16030268
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
S. pupariae is a newly discovered species of parasitoid wasps. Research into its development, behavioral genetics, and molecular mechanisms provides valuable insights for improving integrated pest management strategies. Quantitative real-time PCR (qRT-PCR) is the most commonly used method for analyzing gene expression. This method requires the identification of stable reference genes to accurately evaluate transcriptional level variations. In this study, eight candidate reference genes (TUB, TBP, RPS18, GAPDH, 18S rRNA, RPL32, Actin, and EF1-alpha) were identified and evaluated for their suitability as reference genes. Gene expression levels across different developmental stages were analyzed using three software tools, GeNorm, NormFinder, and BestKeeper, and the online tool RefFinder. The overall ranking of reference gene stability was as follows: RPS18 > 18S rRNA > RPL32 > GAPDH > Actin > TUB > TPB > EF1-alpha. Ultimately, RPS18 was determined to be the most stable reference gene.
引用
收藏
页数：12
相关论文
共 50 条
  • [31] Selection of stable reference genes for quantitative real-time PCR in the Varroa mite, Varroa destructor
    Lee, Joonhee
    Kim, Young H.
    Kim, Kyungmun
    Kim, Dongwon
    Lee, Si H.
    Kim, Sanghyeon
    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2022, 110 (03)
  • [32] Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
    Hoenemann, Claudia
    Hohe, Annette
    ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2011, 14 (01):
  • [33] Validation of reference genes for real-time quantitative RT-PCR studies in Talaromyces marneffei
    Dankai, Wiyada
    Pongpom, Monsicha
    Vanittanakom, Nongnuch
    JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 118 : 42 - 50
  • [34] Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes
    Filatov, M. A.
    Nikishin, D. A.
    Khramova, Y., V
    Semenova, M. L.
    ZYGOTE, 2019, 27 (06) : 392 - 397
  • [35] Selection of reference genes for quantitative real-time PCR analysis in chicken ovary following silver nanoparticle treatment
    Katarzynska-Banasik, Dorota
    Grzesiak, Malgorzata
    Sechman, Andrzej
    ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2017, 56 : 186 - 190
  • [36] Selection of suitable reference genes for quantitative real-time PCR in trabecular meshwork cells under oxidative stress
    Zhao, Jing
    Zhou, HongYan
    Sun, Lixia
    Yang, Ben
    Zhang, Lin
    Shi, Hongfeng
    Zheng, Yajuan
    FREE RADICAL RESEARCH, 2017, 51 (01) : 103 - 111
  • [37] Reference Genes Selection for Quantitative Real-Time PCR Using RankAggreg Method in Different Tissues of Capra hircus
    Najafpanah, Mohammad Javad
    Sadeghi, Mostafa
    Bakhtiarizadeh, Mohammad Reza
    PLOS ONE, 2013, 8 (12):
  • [38] Selection of Stable Reference Genes for Quantitative Real-Time PCR on Herbaceous Peony (Paeonia lactiflora Pall.) in Response to Drought Stress
    Sheng, Zhipeng
    Luan, Yuting
    Xu, Cong
    Tao, Jun
    Zhao, Daqiu
    PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY, 2023, 92 (03) : 801 - 814
  • [39] Selection of Reference Genes for Quantitative Real-Time RT-PCR Studies in Tomato Fruit of the Genotype MT-Rg1
    Gonzalez-Aguilera, Karla L.
    Saad, Carolina F.
    Chavez Montes, Ricardo A.
    Alves-Ferreira, Marcio
    de Folter, Stefan
    FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [40] Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Mythimna loreyi (Lepidoptera: Noctuidae)
    Wang, Liuyang
    Yang, Chaoxia
    Liu, Qingyu
    Zhang, Xiaofang
    Mei, Xiangdong
    Zhang, Tao
    Ning, Jun
    INSECTS, 2024, 15 (03)
12345