Effects of dietary organic selenium, vitamin C, and vitamin E supplementation on growth performance, serum biochemistry, and antioxidant status in juvenile Hypophthalmichthys molitrix

被引：0

作者：

Yu, Hairui ^{[1
]}

Mushtaq, Maida ^{[2
,3
]}

Razzaq, Saira ^{[3
]}

Ali, Umar ^{[3
]}

Khan, Muhammad ^{[2
,4
]}

Rahman, Abdur ^{[1
]}

Quan, Guobo ^{[2
]}

Fatima, Mehroze ^{[3
]}

Naveed, Saima ^{[4
]}

Zafar, Muhammad Hammad ^{[5
]}

Rahman, Muhammad Aziz Ur ^{[6
]}

机构：

[1] Weifang Univ, Weifang Key Lab Coho Salmon Culturing Facil Engn, Key Lab Biochem & Mol Biol, Coll Biol & Oceanog,Inst Modern Facil Fisheries, Weifang, Peoples R China

[2] Yunnan Anim Sci & Vet Inst, Kunming, Yunnan, Peoples R China

[3] Univ Vet & Anim Sci, Dept Zool Fisheries & Aquaculture, Pattoki, Pakistan

[4] Univ Vet & Anim Sci, Dept Anim Nutr, Lahore, Pakistan

[5] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China

[6] Univ Agr Faisalabad, Inst Dairy & Anim Sci, Faisalabad, Pakistan

来源：

FRONTIERS IN MARINE SCIENCE | 2025年 / 12卷

关键词：

juveniles; growth performance; blood analysis; liver enzymes; antioxidants; NUTRITION; PARAMETERS; CHEMISTRY;

D O I：

10.3389/fmars.2025.1555994

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The study aimed to evaluate the effects of dietary supplementation of selenium (Se), vitamin C, and vitamin E on growth performance, meat quality, and antioxidant status in Hypophthalmichthys molitrix juveniles. A total of 480 juveniles (mean weight: 20 +/- 0.29 g) were randomly assigned to 24 aquaria (20 fish per 100 L), which were allocated to eight dietary treatments (three aquaria per treatment) following a completely randomized design. The dietary treatments included: (1) a basal diet without supplementation (Control), (2) selenium supplementation (S), (3) vitamin C supplementation (C), (4) vitamin E supplementation (E), (5) selenium and vitamin C supplementation (SC), (6) selenium and vitamin E supplementation (SE), (7) vitamin C and vitamin E supplementation (EC), and (8) selenium, vitamin C, and vitamin E supplementation (SCE). The supplementation levels were 0.9 mg/kg Se, 300 mg/kg vitamin C, and 100 mg/kg vitamin E, respectively. After a one-week acclimatization period, a 10-week growth trial was conducted. Fish fed the SCE diet exhibited significantly higher (p< 0.05) final weight gain, body length gain, body weight gain percentage, and specific growth rates compared to other treatments. Selenium concentrations in the kidney, liver, pancreas, and muscle were significantly higher (p< 0.05) in the SCE, SC, SE, and EC groups compared to the Control and single-supplement groups. Hematological parameters, including WBC, RBC, HGB, HCT, and MCHC, were significantly higher (p< 0.05) in the SCE group compared to all other groups. Similarly, serum activities of ALT, AST, and ALP were significantly lower (p< 0.05) in the SCE group than in other treatments, while blood glucose levels were unaffected (p > 0.05) by dietary treatments. Antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), were significantly enhanced (p< 0.05) in the whole body and muscle tissues of fish receiving Se, vitamin C, and E supplementation. Thiobarbituric acid reactive substances (TBARS) levels in muscle and serum were significantly higher (p< 0.05) in the Control group compared to all supplemented groups. The proximate composition of meat, including dry matter, fat, ash, and protein contents, was not significantly affected (p > 0.05) by the dietary treatments. In conclusion, dietary supplementation of selenium, vitamin C, and vitamin E significantly improved growth performance, selenium deposition in tissues, and antioxidant status of Hypophthalmichthys molitrix juveniles, without adversely affecting meat chemical composition.

引用

页数：9

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