Transcriptomic Analysis of the Hepatopancreas in the Sex-Related Size Differences of Macrobrachium nipponense

被引:0
|
作者
Wang, Yabing [1 ]
Qiao, Guangde [1 ]
Yue, Yanfeng [1 ]
Peng, Shiming [1 ]
Fu, Hongtuo [2 ]
机构
[1] Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab Marine & Estuarine Fisheries, Minist Agr, Shanghai 200090, Peoples R China
[2] Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr, Wuxi 214081, Peoples R China
关键词
Macrobrachium nipponense; hepatopancreas; RNA-sequencing; sex-related size differences; differentially expressed genes; SINGLE NUCLEOTIDE POLYMORPHISMS; GROWTH-PERFORMANCE; GENE; METABOLISM; TRYPTOPHAN; TRAITS; ASSOCIATION; MECHANISMS; PRAWN; LOCALIZATION;
D O I
10.3390/vetsci11090445
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Macrobrachium nipponense, a commercially popular crustacean species within the Chinese context, is recognized for its exceptional nutritional composition and palatability. There are significant differences in growth between male and female M. nipponense. Herein, transcriptomics was used to determine the hepatopancreas transcriptome differences between sex-related size differences in M. nipponense. We identified 974 differentially expressed genes (DEGs) between the SHE (female) and BHE (male) groups, which were validated using RT-qPCR. The genes encoding matrix metalloproteinase-9 (MM9), Ribosome-binding protein 1 (RBP1), Aly/REF export factor 2, and hematological and neurological expressed 1 (HN1) may play a role in modulating the sex-related size differences observed in M. nipponense. Clusters of orthologous groups and gene ontology functional analysis demonstrated that the DEGs for sex-related size in M.nipponense were associated with various biological functions. The Kyoto Encyclopedia of Genes and Genomes pathways analysis demonstrated that upregulated DEGs were mainly enriched in lysine biosynthesis, tryptophan metabolism, and lysine degradation pathways, whereas the downregulated DEGs were mainly enriched in ascorbate and aldarate metabolism, retinol metabolism, and drug metabolism-cytochrome P450 pathways. The results indicated the molecular mechanism underlying the sex-related size differences and identified key genes. This data will be invaluable to support explanations of individual differences between male and female prawns.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living Status Macrobrachium nipponense in Response to Hypoxia
    Xu, Lei
    Zhang, Wenyi
    Qiao, Hui
    Jiang, Sufei
    Xiong, Yiwei
    Jin, Shubo
    Gong, Yongsheng
    Fu, Hongtuo
    ANTIOXIDANTS, 2022, 11 (01)
  • [2] Identification of Male Sex-Related Genes Regulated by SDHB in Macrobrachium nipponense Based on Transcriptome Analysis after an RNAi Knockdown
    Gao, Zijian
    Zhang, Wenyi
    Jiang, Sufei
    Yuan, Huwei
    Cai, Pengfei
    Jin, Shubo
    Fu, Hongtuo
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (17)
  • [3] Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense
    Jin, S. B.
    Fu, H. T.
    Jiang, S. F.
    Xiong, Y. W.
    Qiao, H.
    Zhang, W. Y.
    Gong, Y. S.
    Wu, Y.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04) : 18396 - 18406
  • [4] Transcriptome analysis of oriental river Prawn(Macrobrachium nipponense) Hepatopancreas in response to ammonia exposure
    Yu, Jielun
    Sun, Jian
    Zhao, Shuo
    Wang, Hui
    Zeng, Qifan
    FISH & SHELLFISH IMMUNOLOGY, 2019, 93 : 223 - 231
  • [5] Genome-wide association and transcriptomic analysis and the identification of growth-related genes in Macrobrachium nipponense
    Gao, Zijian
    Zhang, Wenyi
    Jiang, Sufei
    Qiao, Hui
    Xiong, Yiwei
    Jin, Shubo
    Fu, Hongtuo
    BMC GENOMICS, 2024, 25 (01):
  • [6] Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences
    Yinting Chen
    Christine Lafleur
    Ryan J. Smith
    Diljot Kaur
    Brian T. Driscoll
    Jacqueline C. Bede
    Journal of Chemical Ecology, 2024, 50 : 168 - 184
  • [7] Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences
    Chen, Yinting
    Lafleur, Christine
    Smith, Ryan J.
    Kaur, Diljot
    Driscoll, Brian T.
    Bede, Jacqueline C.
    JOURNAL OF CHEMICAL ECOLOGY, 2024, 50 (3-4) : 168 - 184
  • [8] Sex-related differences in migraine
    Finocchi, Cinzia
    Strada, Laura
    NEUROLOGICAL SCIENCES, 2014, 35 : S207 - S213
  • [9] Sex-related differences in pain
    Cairns, Brian E.
    Gazerani, Parisa
    MATURITAS, 2009, 63 (04) : 292 - 296
  • [10] Sex-related differences in cardiomyopathies
    Pelliccia, Francesco
    Limongelli, Giuseppe
    Autore, Camillo
    Ramon Gimeno-Blanes, Juan
    Basso, Cristina
    Elliott, Perry
    INTERNATIONAL JOURNAL OF CARDIOLOGY, 2019, 286 : 239 - 243