Von Willebrand Factor Collagen-Binding Activity and Von Willebrand Factor-Mediated Platelet Adhesion in Patients with Coronary Artery Disease

被引:0
|
作者
Gabbasov, Zufar [1 ]
Okhota, Sergey [2 ]
Avtaeva, Yuliya [1 ]
Saburova, Olga [1 ]
Melnikov, Ivan [1 ,3 ]
Shtelmakh, Valentina [2 ]
Bazanovich, Sergey [1 ]
Guria, Konstantin [1 ]
Kozlov, Sergey [2 ]
机构
[1] Minist Hlth Russian Federat, Chazov Natl Med Res Ctr Cardiol, Lab Cell Hemostasis, Moscow 121552, Russia
[2] Minist Hlth Russian Federat, Dept Problems Atherosclerosis, Chazov Natl Med Res Ctr Cardiol, Moscow 121552, Russia
[3] Russian Acad Sci, Inst Biomed Problems, State Sci Ctr Russian Federat, Lab Gas Exchange Biomech & Barophysiol, Moscow 123007, Russia
基金
俄罗斯科学基金会;
关键词
von Willebrand factor; collagen; glycoprotein Ib; platelet adhesion; coronary artery disease; ISCHEMIC-HEART-DISEASE; HEMOSTATIC FACTORS; ATHEROSCLEROSIS RISK; FACTOR-VIII; AGE;
D O I
10.3390/biomedicines12092007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we investigated von Willebrand factor (VWF)-related parameters in 30 patients with stable coronary artery disease (CAD) and 50 patients without CAD. In both groups, the following were measured: the VWF antigen level (VWF:Ag); the VWF ristocetin cofactor activity (VWF:RCo); the VWF collagen-binding activity (VWF:CB); and VWF-mediated platelet adhesion. Platelet adhesion was measured in whole blood at a shear rate of 1300 s(-1) using a microfluidic chamber with a collagen-coated surface. VWF:Ag and VWF:RCo were found to be the same in both groups of patients. However, VWF:CB was found to be lower in patients with CAD compared with patients without CAD, with values of 106.7% (82.1; 131.6) and 160.4% (112.5; 218.1), respectively (p < 0.001). The decrease in platelet adhesion after GPIb inhibition was more pronounced in patients with CAD compared with patients of the control group, with recorded values of 76.0% (60.6; 82.1) and 29.3% (0.0; 60.4), respectively (p < 0.001). After adjusting for traditional risk factors, the odds ratio for CAD was found to be 0.98 (95% CI, 0.97-0.99; p = 0.011) per 1% increase in VWF:CB activity, and 1.06 (95% CI, 1.03-1.09; p < 0.001) per 1% decrease in GPIb-mediated platelet adhesion. The findings presented in this paper indicate a possible critical role played by complex VWF-collagen-platelet interactions in the development of CAD.
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页数:10
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