Pseudomonas spirodelae, sp. nov., a bacterium isolated from the roots of the aquatic plant Spirodela polyrhiza

A polyphasic taxonomic study was carried out on strain T5W1T, isolated from the roots of the aquatic plant Spirodela polyrhiza. This isolate is Gramnegative, rod- shaped, motile, aerobic and non- pigmented. Nearly complete 16S rRNA gene sequence homology related the strain to Pseudomonas, with 98.4% similarity to P. guineae, P. peli and P. leptonychotis. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) with the closest phylogenetic neighbour of T5W1T showed differences at the species level, further confirmed by differences in several physiological characteristics. The main fatty acids are summed feature 3 ( C 16:1 omega 7c and/or C 16: 1 omega 6c), C 18: 1 omega 7c, and C 16:0 . The DNA G+C content is 59.3 mol%. Q- 9 is the primary ubiquinone found, and phosphatidylethanolamine is the dominant polar lipid, with lesser amounts of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine. Based on the results obtained, this bacterium is assigned to the genus Pseudomonas as a new species with the name Pseudomonas spirodelae sp. nov., type strain T5W1T (=NRRL B- 65714T =DSM 118085T).