High-speed image reconstruction for nonlinear structured illumination microscopy

被引:0
|
作者
Zhang, Jingxiang [1 ]
Zhao, Tianyu [1 ]
Fu, Xiangda [1 ]
Shu, Manming [1 ]
Yan, Jiajing [1 ]
Wang, Mengrui [1 ]
Liang, Yansheng [1 ]
Wang, Shaowei [1 ]
Lei, Ming [1 ,2 ]
机构
[1] Xi An Jiao Tong Univ, Sch Phys, MOE Key Lab Nonequilibrium Synth & Modulat Condens, Xian 710049, Peoples R China
[2] Xi An Jiao Tong Univ, State Key Lab Elect Insulat & Power Equipment, Xian 710049, Peoples R China
基金
中国国家自然科学基金;
关键词
FLUORESCENCE MICROSCOPY; RESOLUTION ENHANCEMENT; OPTIMIZATION;
D O I
10.1364/PRJ.540671
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
By exploiting the nonlinear responses of fluorescent probes, the spatial resolution of structured illumination microscopy (SIM) can be further increased. However, the traditional reconstruction method of nonlinear structured illumination microscopy (NL-SIM) is very slow due to its complex process, which poses a significant challenge to display super resolution results in real-time. Here, we describe an efficient and robust SIM algorithm that enables rapid and accurate full-process SIM reconstruction. First, we present a fast illumination parameters estimation algorithm based on discrete Fourier transforms that result in a more simplified workflow than that of classical methods. Second, an accelerated NL-SIM reconstruction algorithm is developed by extending a highspeed reconstruction framework, joint space and frequency reconstruction (JSFR), to the NL-SIM. In particular, we provide the open-source MATLAB toolbox of our JSFR-NL-SIM algorithm. The entire image reconstruction process is completed in the milliseconds range, representing a significant time saving for the user. (c) 2025 Chinese Laser Press
引用
收藏
页码:743 / 760
页数:18
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