A novel liquid-liquid phase separation-related gene signature for predicting prognosis in colon cancer

被引：0
作者：
Wang, Shuo [1 ,2 ]
Hou, Sen [1 ,2 ]
Jiang, Shan [3 ]
Wang, Chao [1 ,2 ]
Zhang, Peipei [4 ]
Ye, Yingjiang [1 ,2 ]
Gao, Zhidong [1 ,2 ]
机构：
[1] Peking Univ, Peoples Hosp, Dept Gastroenterol Surg, Beijing, Peoples R China
[2] Peking Univ, Peoples Hosp, Lab Surg Oncol, Beijing, Peoples R China
[3] Shandong First Med Univ, Shandong Prov Hosp, Dept Gastroenterol, Jinan, Shandong, Peoples R China
[4] Peking Univ, Natl Hlth & Family Planning Commiss, Sch Basic Med Sci,Minist Educ,Dept Biochem, Key Laboratory for Neurosci,Hlth Sci Ctr, Beijing, Peoples R China
来源：
FRONTIERS IN IMMUNOLOGY | 2024年 / 15卷
基金：
中国国家自然科学基金;
关键词：
liquid-liquid phase separation; colon cancer; prognosis; immunotherapy; nomogram; TUMOR-SUPPRESSOR; RESISTANCE;
D O I：
10.3389/fimmu.2024.1514613
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Background An increasing body of evidence indicates that dysregulation of liquid-liquid phase separation (LLPS) in cellular processes is implicated in the development of diverse tumors. Nevertheless, the association between LLPS and the prognosis, as well as the tumor immune microenvironment, in individuals with colon cancer remains poorly understood.Methods We conducted a comprehensive evaluation of the LLPS cluster in 1010 colon cancer samples from the TCGA and GEO databases, utilizing the expression profiles of LLPS-related prognostic differentially expressed genes (DEGs). Subsequently, a LLPS-related gene signature was constructed to calculate the LLPS-related risk score (LRRS) for each individual patient.Results Two LLPS subtypes were identified. Substantial variations were observed between the two LLPS subtypes in terms of prognosis, pathway activity, clinicopathological characteristics, and immune characteristics. Patients with high LRRS exhibited worse prognosis and poorer response to immunotherapy. LRRS was found to be correlated with the clinicopathological characteristics, genomic alterations, and the potential response to immune checkpoint inhibitors therapy of colon cancer patients. Additionally, the biological function of a key gene POU4F1 was verified in vitro.Conclusions This study highlights the crucial role of LLPS in colon cancer, LRRS can be used to predict the prognosis of colon cancer patients and aid in the identification of more effective immunotherapy strategies.
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页数：18
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