Synthesis and Characterization of Alginate Microparticles for Oral Delivery of Alpha-Tocopherol

被引:0
作者
Zivkovic, Milja [1 ]
Barjaktarevic, Ana [1 ]
Tomovic, Marina [1 ]
Ustevic, Andela [1 ]
机构
[1] Univ Kragujevac, Fac Med Sci, Dept Pharm, Kragujevac, Serbia
关键词
alpha-tocopherol; vitamin E; calcium alginate; alginate particles; encapsulation; CROSS-LINKING; DRUG;
D O I
10.5937/afmnai41-46914
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Introduction/Aim. Microencapsulation technology can be used for the protection of alpha-tocopherol from degradation in unfavorable environments and enhancement of bioavailability and shelf-life of vitamin E. The aim of this study was the synthesis and characterization of alginate microparticles for the oral delivery of alpha-tocopherol. Methods. Four different formulations of alpha-tocopherol loaded calcium alginate microparticles for oral delivery were synthesized by external ionotropic gelation method. The vitamin E content in microparticles was 0.5%, 1% and 2% (w/w); the vitamin E/sodium alginate ratio was 1:1 and 1:2. All microparticles were characterized by average particles size, swelling degree, vitamin E content, loading capacity, and encapsulation efficiency. Results. Spherically shaped microparticles with the diameter of 500 to 1000 mu m were obtained after the drying process. The size and the swelling degree did not change significantly in 0.1 M HCL, while they were increased in base conditions of phosphate buffer of pH 6.8 and 7.4. Encapsulated vitamin E content was not significantly different between formulations (0.30 +/- 0.010 - 0.60 +/- 0.021 mg/mL). The loading capacities were in the range between 10 +/- 0.11% and 20.45 +/- 0.22%, while encapsulation efficiency percentages were between 18.94 +/- 0.32% and 31.91 +/- 0.41%. Conclusion. The optimum conditions for alpha-tocopherol encapsulation with the highest percentage of loading capacity and encapsulation efficacy were obtained using 1% sodium alginate, 2% calcium chloride, and vitamin E/polymer in the ratio 1:1. All four formulations showed the expected behavior in different mediums, which simulated gastrointestinal fluids in vivo (0.1 M HCL, phosphate buffer pH 6.8 and pH 7.4): gastroresistance, increasing in the size, and swelling degree in intestinal fluids. This emphasizes the use of alginate microparticles as a carrier for the oral delivery of vitamin E.
引用
收藏
页码:396 / 405
页数:10
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