Integrated proteomics and metabolomics analyses reveal the mechanism of calcium ascorbate inhibiting tobacco browning

被引:0
作者
Yang, Wenjuan [1 ]
Sun, Jingguo [2 ]
Chen, Zhenguo [2 ]
Feng, Ji [2 ]
Li, Jianping [2 ]
Yang, Yong [1 ]
Sun, Guangwei [2 ]
Xiang, Haibo [1 ]
机构
[1] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China
[2] Tobacco Res Inst Hubei Prov, Wuhan 430030, Hubei, Peoples R China
关键词
Browning; Tobacco; Calcium ascorbate; Proteomics; Metabolomics; NICOTINE; STRESS; COLOR; FRUIT; ACID;
D O I
10.1016/j.indcrop.2025.120763
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Flue-cured tobacco often undergoes browning, which not only diminishes its commercial value but also increases potential health risks for smokers. Previous researches have demonstrated the potential of calcium ascorbate (CAAS) in preventing browning in fruits and vegetables. However, its efficacy in mitigating browning in tobacco leaf and the associated molecular mechanisms have not been extensively investigated. This study established a classification model for browning degree in tobacco leaves based on CIE color parameters and a multilayer perceptron neural network. In addition to evaluating the antioxidant effects of CAAS on tobacco leaves during the flue-curing process, we conducted integrated proteomic and metabolomic profiling to elucidate the molecular mechanisms by which CAAS prevents browning. The findings indicated that the overall accuracy of the classification model was above 94 %. According to the prediction results of the model, considering the physical characteristics, sensory attributes, economic significance, and chemical composition of the flue-cured leaves, 0.2 % CAAS was identified as the most effective inhibitor of browning in tobacco leaves. The mechanisms by which CAAS prevents browning indicated that it not only accelerated the yellowing process and enhanced antioxidant activity, but also inhibited enzymatic browning reactions by increasing POD activity and carotenoid content, while reducing the activity of PPO and the levels of MDA, chlorophyll, and H2O2. Furthermore, a combination of proteomic and metabolomic analysis showed that CAAS could upregulate proteins involved in photosynthesis while downregulating metabolites involved in purine metabolism, phenylpropanoid biosynthesis, and tyrosine metabolism, thereby further mitigating browning. The results of this study provide new insights into the mechanisms by which CAAS inhibits tobacco browning and suggest potential strategies for its application in the tobacco industry.
引用
收藏
页数:15
相关论文
共 50 条
[41]   Different color regulation mechanism in willow barks determined using integrated metabolomics and transcriptomics analyses [J].
Zhou, Jie ;
Guo, Jiahui ;
Chen, Qingsheng ;
Wang, Baosong ;
He, Xudong ;
Zhuge, Qiang ;
Wang, Pu .
BMC PLANT BIOLOGY, 2022, 22 (01)
[42]   Integrated proteomics and metabolomics analyses reveal new insights into the antitumor effects of valproic acid plus simvastatin combination in a prostate cancer xenograft model associated with downmodulation of YAP/TAZ signaling [J].
Iannelli, Federica ;
Lombardi, Rita ;
Costantini, Susan ;
Roca, Maria Serena ;
Addi, Laura ;
Bruzzese, Francesca ;
Di Gennaro, Elena ;
Budillon, Alfredo ;
Pucci, Biagio .
CANCER CELL INTERNATIONAL, 2024, 24 (01)
[43]   Integrated Proteomics and Metabolomics Analyses of Serum in Chinese Patients with Severe and Active Graves' Orbitopathy: A Cross-sectional Study [J].
Shi, Ting-Ting ;
Liu, Hong-Ying ;
Zhu, Xiao-Rong ;
Xin, Zhong ;
Hua, Lin ;
Xie, Rong-Rong ;
Sun, Ran ;
Cao, Xi ;
Yang, Jin- Kui .
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS, 2023, 23 (09) :1151-1161
[44]   Integrated microbiology and metabolomics analysis reveal the fermentation process and the flavor development in cigar tobacco leaf [J].
Zhang, Guanghai ;
He, Yue ;
Yang, Wanlong ;
Liu, Ziyi ;
Lin, Zhonglong ;
Zhang, Tikun ;
He, Xiaohui ;
Xia, Huachan ;
Huo, Mengting ;
Yao, Heng ;
Zhao, Gaokun ;
Wu, Yuping ;
Kong, Guanghui .
MICROBIOLOGY SPECTRUM, 2025, 13 (06)
[45]   Integrated physiological and omics analyses reveal the mechanism of beneficial fungal Trichoderma sp. alleviating cadmium toxicity in tobacco (Nicotiana tabacum L.) [J].
Zhang, Shuo ;
Zhang, Chulong ;
Gao, Zi-Feng ;
Qiu, Cheng-Wei ;
Shi, Shou-Heng ;
Chen, Zhong-Hua ;
Ali, Mohamed Abdelalim ;
Wang, Feng ;
Wu, Feibo .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2023, 267
[46]   Combined transcriptomics and metabolomics analyses reveal the molecular mechanism of heat tolerance in Pichia kudriavzevii [J].
Wang, Ning ;
Li, Lu ;
Ma, Yi ;
Shen, Caihong ;
Ao, Zonghua ;
Song, Chuan ;
Mehmood, Muhammad Aamer ;
Zhang, Puyu ;
Liu, Ying ;
Sun, Xiaoke ;
Zhu, Hui .
FRONTIERS IN MICROBIOLOGY, 2025, 16
[47]   Integrated microbiological and metabolomics analyses to understand the mechanism that allows modified biochar to affect the alkalinity of saline soil and winter wheat growth [J].
Duan, Manli ;
Yan, Rupan ;
Wang, Quanjiu ;
Zhou, Beibei ;
Zhu, Hongyan ;
Liu, Guohuan ;
Guo, Xu ;
Zhang, Zhenshi .
SCIENCE OF THE TOTAL ENVIRONMENT, 2023, 866
[48]   Combined metabolomics and proteomics to reveal the mechanism of S. oneidensis MR-1 degradation malathion enhanced by FeO/C [J].
Zhang, Jie ;
Tang, Shen ;
Li, Yanhong ;
Zhang, Jing ;
Peng, Yuqing ;
Peng, Jingjing ;
Cen, Yu ;
Shi, Peizhu .
JOURNAL OF HAZARDOUS MATERIALS, 2024, 476
[49]   Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection [J].
Li, Mengyuan ;
Yang, Jiali ;
Ye, Chuantao ;
Bian, Peiyu ;
Yang, Xiaofei ;
Zhang, Haijun ;
Luo, Chuanyu ;
Xue, Zhifeng ;
Lei, Yingfeng ;
Lian, Jianqi .
VIROLOGICA SINICA, 2021, 36 (06) :1554-1565
[50]   Proteomics and metabolomics analysis reveal potential mechanism of extended-spectrum β-lactamase production inEscherichia coli [J].
Ma, He ;
Lai, Bingjie ;
Jin, Yufen ;
Tian, Chang ;
Liu, Jiaying ;
Wang, Ke .
RSC ADVANCES, 2020, 10 (45) :26862-26873