Mouse oligoadenylate synthetase 1b inhibits West Nile virus replication by acting through the stem- loop 2 in the 3′-untranslated region

被引：0
作者：
Sato, Keisuke [1 ]
Nakamura, Teppei [1 ]
Morimatsu, Masami [1 ]
Agui, Takashi [1 ]
机构：
[1] Hokkaido Univ, Fac Vet Med, Lab Lab Anim Sci & Med, Sapporo 0600818, Japan
来源：
JAPANESE JOURNAL OF VETERINARY RESEARCH | 2024年 / 72卷 / 02期
关键词：
Inhibition of virus replication; Oligoadenylate synthetase; West Nile virus; 2'; 5'-OLIGOADENYLATE SYNTHETASE; RNASE-L; FLAVIVIRUS; GENES; SUSCEPTIBILITY; RESISTANT; ISOFORM; GENOME; OAS1B;
D O I：
10.57494/jjvr.72.2_65
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Oligoadenylate synthetase (OAS) plays a role in intracellular viral defense. Mouse Oas1b is responsible for the susceptibility to flaviviruses. The flavivirus replication inhibitory effect of Oas1b is independent of its enzymatic activity; however, its mechanism remains unclear, though mouse Oas1b possibly targets the 3 '-stem-loops (SLs). We measured the effect of Oas1b by removing and overexpressing SLs in the 3 '-untranslated regions (UTRs) of the West Nile virus (WNV) replicon. Both SL2 deletion and SL2 RNA overexpression in the 3 '-UTR of the WNV replicon resulted in the loss of Oas1b-mediated replication inhibition. These results revealed that WNV SL2 is essential for Oas1b-mediated replication inhibition.
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页码：65 / 70
页数：6
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