Mouse oligoadenylate synthetase 1b inhibits West Nile virus replication by acting through the stem- loop 2 in the 3′-untranslated region

被引：0
作者：
Sato, Keisuke [1 ]
Nakamura, Teppei [1 ]
Morimatsu, Masami [1 ]
Agui, Takashi [1 ]
机构：
[1] Hokkaido Univ, Fac Vet Med, Lab Lab Anim Sci & Med, Sapporo 0600818, Japan
来源：
JAPANESE JOURNAL OF VETERINARY RESEARCH | 2024年 / 72卷 / 02期
关键词：
Inhibition of virus replication; Oligoadenylate synthetase; West Nile virus; 2'; 5'-OLIGOADENYLATE SYNTHETASE; RNASE-L; FLAVIVIRUS; GENES; SUSCEPTIBILITY; RESISTANT; ISOFORM; GENOME; OAS1B;
D O I：
10.57494/jjvr.72.2_65
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Oligoadenylate synthetase (OAS) plays a role in intracellular viral defense. Mouse Oas1b is responsible for the susceptibility to flaviviruses. The flavivirus replication inhibitory effect of Oas1b is independent of its enzymatic activity; however, its mechanism remains unclear, though mouse Oas1b possibly targets the 3 '-stem-loops (SLs). We measured the effect of Oas1b by removing and overexpressing SLs in the 3 '-untranslated regions (UTRs) of the West Nile virus (WNV) replicon. Both SL2 deletion and SL2 RNA overexpression in the 3 '-UTR of the WNV replicon resulted in the loss of Oas1b-mediated replication inhibition. These results revealed that WNV SL2 is essential for Oas1b-mediated replication inhibition.
引用
收藏
页码：65 / 70
页数：6
相关论文
共 28 条
  • [1] Conserved Active-Site Residues Associated with OAS Enzyme Activity and Ubiquitin-Like Domains Are Not Required for the Antiviral Activity of goOASL Protein against Avian Tembusu Virus
    Chen, Shun
    Yang, Chao
    Zhang, Jinyue
    Wu, Zhen
    Wang, Mingshu
    Jia, Renyong
    Zhu, Dekang
    Liu, Mafeng
    Yang, Qiao
    Wu, Ying
    Zhao, Xinxin
    Zhang, Shaqiu
    Liu, Yunya
    Zhang, Ling
    Yu, Yanling
    You, Yu
    Cheng, Anchun
    [J]. VIRUSES-BASEL, 2018, 10 (07):
  • [2] The conserved stem-loop II structure at the 3' untranslated region of Japanese encephalitis virus genome is required for the formation of subgenomic flaviviral RNA
    Chen, Yi-Shiuan
    Fan, Yi-Hsin
    Tien, Chih-Feng
    Yueh, Andrew
    Chang, Ruey-Yi
    [J]. PLOS ONE, 2018, 13 (07):
  • [3] Functional non-coding RNAs derived from the flavivirus 3′ untranslated region
    Clarke, B. D.
    Roby, J. A.
    Slonchak, A.
    Khromykh, A. A.
    [J]. VIRUS RESEARCH, 2015, 206 : 53 - 61
  • [4] Characterization of the termini of the West Nile virus genome and their interactions with the small isoform of the 2′ 5′-oligoadenylate synthetase family
    Deo, Soumya
    Patel, Trushar R.
    Chojnowski, Grzegorz
    Koul, Amit
    Dzananovic, Edis
    McEleney, Kevin
    Bujnicki, Janusz M.
    McKenna, Sean A.
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2015, 190 (02) : 236 - 249
  • [5] The Flvr-encoded murine oligoadenylate synthetase 1b (Oas1b) suppresses 2-5A synthesis in intact cells
    Elbahesh, H.
    Jha, B. K.
    Silverman, R. H.
    Scherbik, S. V.
    Brinton, M. A.
    [J]. VIROLOGY, 2011, 409 (02) : 262 - 270
  • [6] The role of mouse 2′,5′-oligoadenylate synthetase 1 paralogs
    Elkhateeb, Enas
    Tag-El-Din-Hassan, Hassan T.
    Sasaki, Nobuya
    Torigoe, Daisuke
    Morimatsu, Masami
    Agui, Takashi
    [J]. INFECTION GENETICS AND EVOLUTION, 2016, 45 : 393 - 401
  • [7] Human Genetic Variants Associated with COVID-19 Severity are Enriched in Immune and Epithelium Regulatory Networks
    Feng, Zhanying
    Ren, Xianwen
    Duren, Zhana
    Wang, Yong
    [J]. PHENOMICS, 2022, 2 (06): : 389 - 403
  • [8] Genetic Diversity in the Collaborative Cross Model Recapitulates Human West Nile Virus Disease Outcomes
    Graham, Jessica B.
    Thomas, Sunil
    Swarts, Jessica
    McMillan, Aimee A.
    Ferris, Martin T.
    Suthar, Mehul S.
    Treuting, Piper M.
    Ireton, Renee
    Gale, Michael, Jr.
    Lund, Jennifer M.
    [J]. MBIO, 2015, 6 (03): : 1 - 11
  • [9] The 2′,5′-oligoadenylate synthetase 1b is a potent inhibitor of West Nile virus replication inside infected cells
    Kajaste-Rudnitski, A
    Mashimo, T
    Frenkiel, MP
    Guénet, JL
    Lucas, M
    Desprès, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (08) : 4624 - 4637
  • [10] The Oligoadenylate Synthetase Family: An Ancient Protein Family with Multiple Antiviral Activities
    Kristiansen, Helle
    Gad, Hans Henrik
    Eskildsen-Larsen, Signe
    Despres, Philippe
    Hartmann, Rune
    [J]. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2011, 31 (01) : 41 - 47
123