Establishment of a platform imaged capillary isoelectric focusing (icIEF) characterization method for adeno-associated virus (AAV) capsid proteins

被引：12

作者：

Wu, Jiaqi ^{[1
]}

Heger, Chris ^{[1
]}

机构：

[1] ProteinSimple, San Jose, CA 95134 USA

来源：

GREEN ANALYTICAL CHEMISTRY | 2022年 / 3卷

关键词：

D O I：

10.1016/j.greeac.2022.100027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein we describe a platform method to study the charge heterogeneity of adeno-associated virus (AAV) capsid proteins. The method, based on imaged capillary isoelectric focusing (icIEF) with native fluorescence imaging detection, was validated, and can be used as an identity assay and stability indication assay. The protocol was also evaluated following the White Analytical Chemistry principle, its score is 89.7. The method is shown to be platform, with eight different AAV serotypes successfully analyzed. The resulting AAV serotype 'fingerprints' can be used for AAV identity and stability indication.

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页数：6

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