Histopathological and immunohistochemical examination of the brains of rabid dogs in the Philippines

被引：0

作者：

Markbordee, Boonkanit ^{[1
]}

Cabic, Alpha Grace B. ^{[2
]}

Iamohbhars, Nuttipa ^{[1
]}

Shiwa-sudo, Nozomi ^{[3
]}

Kimitsuki, Kazunori ^{[4
]}

Espino, Mark Joseph M. ^{[2
]}

Nacion, Leilanie B. ^{[2
]}

Manalo, Daria Llenarresas ^{[2
]}

Inoue, Satoshi ^{[1
,5
]}

Park, Chun-Ho ^{[1
]}

机构：

[1] Kitasato Univ, Sch Vet Med, Dept Vet Pathol, 23-35-1 Higashi, Aomori 0348628, Japan

[2] Res Inst Trop Med, Dept Hlth, Muntinlupa, Philippines

[3] Natl Inst Infect Dis, Dept Pathol, Tokyo, Japan

[4] Oita Univ, Fac Med, Dept Microbiol, Oita, Japan

[5] Natl Inst Infect Dis, Dept Vet Sci, Tokyo, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2024年 / 86卷 / 12期

基金：

日本学术振兴会;

关键词：

brain; histopathology; immunohistochemistry; Philippines; rabid dog; MOLECULAR EPIDEMIOLOGY; VIRUS; TRANSCRIPTION; PATHOGENESIS; DIAGNOSIS; ANTIGEN; BODIES; SITES;

D O I：

10.1292/jvms.24-0249

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

. Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity.

引用

页码：1243 / 1251

页数：9

共 45 条

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