Identification and expression analysis of calcium-dependent protein kinase family in oat (Avena sativa L.) and their functions in response to saline-alkali stresses

被引：0

作者：

Li, Ya-nan ^{[1
]}

Lei, Chunyan ^{[1
]}

Yang, Qian ^{[1
]}

Yu, Xiao ^{[1
]}

Li, Siming ^{[1
]}

Sun, Yan ^{[1
]}

Ji, Chunli ^{[1
]}

Zhang, Chunhui ^{[1
]}

Xue, Jin-ai ^{[1
]}

Cui, Hongli ^{[2
]}

Li, Runzhi ^{[1
]}

机构：

[1] Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Coll Agr, Jinzhong, Shanxi, Peoples R China

[2] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandon, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2024年 / 15卷

基金：

中国国家自然科学基金;

关键词：

oat (Avena sativa L.); saline-alkali stresses; calcium-dependent protein kinase (CDPK); Chlamydomonas reinhardtii; genetic transformation; Na plus / H plus antiporter 1 (NHX1); PLASMA-MEMBRANE; SIGNAL-TRANSDUCTION; CHLAMYDOMONAS-REINHARDTII; OVER-EXPRESSION; SALT TOLERANCE; RICE; DROUGHT; TRANSCRIPTION; CONFERS; COLD;

D O I：

10.3389/fpls.2024.1395696

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Calcium-dependent protein kinases (CDPKs) serve as calcium ion sensors and play crucial roles in all aspects of plant life cycle. While CDPK gene family has been extensively studied in various plants, there is limited information available for CDPK members in oat, an important cereal crop worldwide. Totally, 60 AsCDPK genes were identified in oat genome and were classified into four subfamilies based on their phylogenetic relationship. The members within each subfamily shared similar gene structure and conserved motifs. Collinearity analysis revealed that AsCDPK gene amplification was attributed to segmental duplication events and underwent strong purifying selection. AsCDPK promoters were predicted to contain cis-acting elements associated with hormones, biotic and abiotic stresses. AsCDPK gene expressions were induced by different salt stresses, exhibiting stress-specific under different salt treatments. Moreover, overexpression of AsCDPK26 gene enhanced salt resistance in C. reinhardtii, a single-cell photoautotrophic model plants. Further analysis revealed a significant correlation between AsCDPK26 and Na+/H+ antiporter 1 (p<0.05), suggesting that AsCDPK26 may interact with ion transporter to modulate salt resistance. These results not only provide valuable insights into AsCDPK genes in response to different salt stresses, but also lay the foundation to mine novel candidates for improving salt tolerance in oat and other crops.

引用

页数：22

共 36 条

[21] Molecular cloning and expression analysis of eight calcium-dependent protein kinase (CDPK) genes from banana (Musa acuminata L. AAA group, cv. Cavendish) [J].

Wang, Z. ;

Li, J. ;

Jia, C. ;

Xu, B. ;

Jin, Z. .

SOUTH AFRICAN JOURNAL OF BOTANY, 2016, 104 :134-141

[22] Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp. indica) [J].

Shuifeng Ye ;

Lei Wang ;

Weibo Xie ;

Bingliang Wan ;

Xianghua Li ;

Yongjun Lin .

Plant Molecular Biology, 2009, 70 :311-325

[23] Genome-wide identification and expression analysis of the response regulator gene family in alfalfa (Medicago sativa L.) reveals their multifarious roles in stress response [J].

Qiang, Yuqin ;

He, Xiaojuan ;

Li, Zhen ;

Li, Siqi ;

Zhang, Jia ;

Liu, Tao ;

Tursunniyaz, Mamateliy ;

Wang, Xinyu ;

Liu, Zhipeng ;

Fang, Longfa .

FRONTIERS IN PLANT SCIENCE, 2023, 14

[24] Genome-Wide Identification, Characterization, and Expression Analysis under Abiotic Stresses of the UBP Gene Family in Rice (Oryza sativa L.) [J].

Zou, Xiaoxiao ;

Li, Yongliang ;

Yin, Huangping ;

Xu, Jiajin ;

Li, Zeqi ;

Jiang, Shuai ;

Chen, Fenglin ;

Li, You ;

Xiao, Wenjun ;

Liu, Shucan ;

Guo, Xinhong .

AGRONOMY-BASEL, 2023, 13 (11)

[25] Genome-wide identification, classification, and expression analysis of the arabinogalactan protein gene family in rice (Oryza sativa L.) [J].

Ma, Haoli ;

Zhao, Jie .

JOURNAL OF EXPERIMENTAL BOTANY, 2010, 61 (10) :2647-2668

[26] Genome-wide analysis of calcium-dependent protein kinase (CDPK) family and functional characterization of TaCDPK25-U in response to drought stress in wheat [J].

Linghu, Bin ;

Xu, Zhuoling ;

Chu, Yingqi ;

Yan, Ying ;

Nie, Xiaojun ;

Weining, Song .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2023, 209

[27] Genome-wide identification, evolution analysis of LysM gene family members and their expression analysis in response to biotic and abiotic stresses in banana (Musa L.) [J].

Ren, Wenhui ;

Zhang, Chengyu ;

Wang, Mengge ;

Zhang, Chunyu ;

Xu, Xiaoqiong ;

Huang, Yuji ;

Chen, Yukun ;

Lin, Yuling ;

Lai, Zhongxiong .

GENE, 2022, 845

[28] Genome-Wide Identification and Expression Analysis of RCC1 Gene Family under Abiotic Stresses in Rice (Oryza sativa L.) [J].

Cen, Qiwen ;

Kang, Lihua ;

Zhou, Danni ;

Zhang, Xian ;

Tian, Quanxiang ;

Zhang, Xiaoqin ;

Mou, Wangshu ;

Dang, Cong ;

Fang, Yunxia ;

Xue, Dawei .

AGRONOMY-BASEL, 2023, 13 (03)

[29] Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families:: Comprehensive analysis of the CDPKs gene family in rice [J].

Asano, T ;

Tanaka, N ;

Yang, GX ;

Hayashi, N ;

Komatsu, S .

PLANT AND CELL PHYSIOLOGY, 2005, 46 (02) :356-366

[30] Genome-Wide Identification and Expression Analysis of MYB Transcription Factor Family in Response to Various Abiotic Stresses in Coconut (Cocos nucifera L.) [J].

Si, Cheng-Cheng ;

Li, Yu-Bin ;

Hai, Xue ;

Bao, Ci-Ci ;

Zhao, Jin-Yang ;

Ahmad, Rafiq ;

Li, Jing ;

Wang, Shou-Chuang ;

Li, Yan ;

Yang, Yao-Dong .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (18)

← 1 2 3 4 →