Identification and expression analysis of calcium-dependent protein kinase family in oat (Avena sativa L.) and their functions in response to saline-alkali stresses

被引:0
|
作者
Li, Ya-nan [1 ]
Lei, Chunyan [1 ]
Yang, Qian [1 ]
Yu, Xiao [1 ]
Li, Siming [1 ]
Sun, Yan [1 ]
Ji, Chunli [1 ]
Zhang, Chunhui [1 ]
Xue, Jin-ai [1 ]
Cui, Hongli [2 ]
Li, Runzhi [1 ]
机构
[1] Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Coll Agr, Jinzhong, Shanxi, Peoples R China
[2] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandon, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2024年 / 15卷
基金
中国国家自然科学基金;
关键词
oat (Avena sativa L.); saline-alkali stresses; calcium-dependent protein kinase (CDPK); Chlamydomonas reinhardtii; genetic transformation; Na plus / H plus antiporter 1 (NHX1); PLASMA-MEMBRANE; SIGNAL-TRANSDUCTION; CHLAMYDOMONAS-REINHARDTII; OVER-EXPRESSION; SALT TOLERANCE; RICE; DROUGHT; TRANSCRIPTION; CONFERS; COLD;
D O I
10.3389/fpls.2024.1395696
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Calcium-dependent protein kinases (CDPKs) serve as calcium ion sensors and play crucial roles in all aspects of plant life cycle. While CDPK gene family has been extensively studied in various plants, there is limited information available for CDPK members in oat, an important cereal crop worldwide. Totally, 60 AsCDPK genes were identified in oat genome and were classified into four subfamilies based on their phylogenetic relationship. The members within each subfamily shared similar gene structure and conserved motifs. Collinearity analysis revealed that AsCDPK gene amplification was attributed to segmental duplication events and underwent strong purifying selection. AsCDPK promoters were predicted to contain cis-acting elements associated with hormones, biotic and abiotic stresses. AsCDPK gene expressions were induced by different salt stresses, exhibiting stress-specific under different salt treatments. Moreover, overexpression of AsCDPK26 gene enhanced salt resistance in C. reinhardtii, a single-cell photoautotrophic model plants. Further analysis revealed a significant correlation between AsCDPK26 and Na+/H+ antiporter 1 (p<0.05), suggesting that AsCDPK26 may interact with ion transporter to modulate salt resistance. These results not only provide valuable insights into AsCDPK genes in response to different salt stresses, but also lay the foundation to mine novel candidates for improving salt tolerance in oat and other crops.
引用
收藏
页数:22
相关论文
共 36 条
  • [1] Genome-wide identification and expression analysis of calcium-dependent protein kinase in maize
    Kong, Xiangpei
    Lv, Wei
    Jiang, Shanshan
    Zhang, Dan
    Cai, Guohua
    Pan, Jiaowen
    Li, Dequan
    BMC GENOMICS, 2013, 14
  • [2] Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
    Fedorowicz-Stronska, Olga
    Koczyk, Grzegorz
    Kaczmarek, Malgorzata
    Krajewski, Pawel
    Sadowski, Jan
    JOURNAL OF APPLIED GENETICS, 2017, 58 (01) : 11 - 22
  • [3] Genome-wide survey and expression analysis of the calcium-dependent protein kinase gene family in cassava
    Hu, Wei
    Hou, Xiaowan
    Xia, Zhiqiang
    Yan, Yan
    Wei, Yunxie
    Wang, Lianzhe
    Zou, Meiling
    Lu, Cheng
    Wang, Wenquan
    Peng, Ming
    MOLECULAR GENETICS AND GENOMICS, 2016, 291 (01) : 241 - 253
  • [4] Genome-wide identification and characterization of Calcium-Dependent Protein Kinase (CDPK) gene family in autotetraploid cultivated alfalfa (Medicago sativa subsp. sativa) and expression analysis under abiotic stresses
    Han, Bingcheng
    Dong, Xueming
    Shi, Congcong
    Wang, Zhaoming
    Chen, Jiwei
    Li, Pengzhen
    Yan, Wei
    Zhou, Qiang
    Liu, Zhipeng
    Yan, Longfeng
    BMC PLANT BIOLOGY, 2024, 24 (01):
  • [5] Genome-wide identification of the calcium-dependent protein kinase gene family in Fragaria vesca and expression analysis under different biotic stresses
    Xiong, Yifan
    Lin, Dahe
    Ma, Shiwei
    Wang, Chunhua
    Lin, Shoukai
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2022, 164 (02) : 283 - 298
  • [6] Identification, expression and interaction analyses of calcium-dependent protein kinase (CPK) genes in canola (Brassica napus L.)
    Zhang, Hanfeng
    Liu, Wu-Zhen
    Zhang, Yupeng
    Deng, Min
    Niu, Fangfang
    Yang, Bo
    Wang, Xiaoling
    Wang, Boya
    Liang, Wanwan
    Deyholos, Michael K.
    Jiang, Yuan-Qing
    BMC GENOMICS, 2014, 15
  • [7] Identification and expression assay of calcium-dependent protein kinase family genes in Hevea brasiliensis and determination of HbCDPK5 functions in disease resistance
    Zhang, Bei
    Song, Yufeng
    Zhang, Xiaodong
    Wang, Qiannan
    Li, Xiuqiong
    He, Chaozu
    Luo, Hongli
    TREE PHYSIOLOGY, 2022, 42 (05) : 1070 - 1083
  • [8] Genome-wide identification and expression analysis of calcium-dependent protein kinase and its related kinase gene families in melon (Cucumis melo L.)
    Zhang, Haifei
    Wei, Chunhua
    Yang, Xiaozhen
    Chen, Hejie
    Yang, Yongchao
    Mo, Yanling
    Li, Hao
    Zhang, Yong
    Ma, Jianxiang
    Yang, Jianqiang
    Zhang, Xian
    PLOS ONE, 2017, 12 (04):
  • [9] Genome-wide identification of AAAP gene family and expression analysis in response to saline-alkali stress in foxtail millet (Setaria italica L.)
    Wang, Huimin
    Li, Yun
    Guo, Zhenqing
    Zhou, Xiaoke
    Zhao, Yuxue
    Han, Yucui
    Lin, Xiaohu
    SCIENTIFIC REPORTS, 2024, 14 (01)
  • [10] Genome-wide identification and characterization of the C2 domain family in Sorghum bicolor (L.) and expression profiles in response to saline-alkali stress
    Niu, Jiangshuai
    Li, Zhijiang
    Zhu, Jiarui
    Wu, Rong
    Kong, Lingxin
    Niu, Tingli
    Li, Xueying
    Cheng, Xinran
    Li, Jianying
    Dai, Lingyan
    PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2022, 28 (09) : 1695 - 1711