Nanosecond Molecular Motion in pHP1α Liquid-Liquid Phase Separation Captured by Solid-State NMR

被引:0
|
作者
Chin, Sze Yuet [1 ]
Zhao, Lei [2 ]
Chen, Yinglu [2 ,3 ]
Zhai, Ziwei [2 ,3 ]
Shi, Xiangyan [2 ]
Xue, Kai [1 ,4 ]
机构
[1] Nanyang Technol Univ, Ctr High Field NMR Spect & Imaging, Singapore 637371, Singapore
[2] Shenzhen MSU BIT Univ, Dept Biol, Shenzhen 518172, Guangdong Provi, Peoples R China
[3] Lomonosov Moscow State Univ, Dept Biol, Moscow 119234, Russia
[4] Nanyang Technol Univ, Sch Phys & Math Sci, Singapore 637371, Singapore
来源
JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2025年 / 16卷 / 04期
基金
中国国家自然科学基金;
关键词
MODEL-FREE APPROACH; DYNAMICS; RELAXATION; RESOLUTION; PROTEINS;
D O I
10.1021/acs.jpclett.4c03331
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The relationship among protein structure, function, and dynamics is fundamental to biological activity, particularly in more complex biomolecular systems. Solid-state and solution-state NMR techniques offer powerful means to probe these dynamics across various time scales. However, standard assumptions about molecular motion are often challenged in phase-separated systems like phosphorylated heterochromatin protein 1 alpha (pHP1 alpha), which exhibit both solid- and solution-like characteristics. This study investigates the nanosecond molecular motions in pHP1 alpha liquid-liquid phase separation (LLPS) using relaxation in hetNOE-filtered HSQC signals. By systematically analyzing motions captured by hetNOE-filtered HSQC and conventional HSQC, we characterize the global dynamics site-specifically in pHP1 alpha LLPS. Our findings reveal similar to 15 ns motion in the pHP1 alpha LLPS system, suggesting the coexistence of different dynamic phases, and support previous observations on its role in chromatin organization. This work contributes to the expanding literature on phase-separated biomolecular behavior, with implications for understanding the molecular basis of chromatin compaction and genomic stability.
引用
收藏
页码:1150 / 1156
页数:7
相关论文
共 50 条
  • [1] Investigating Different Dynamic pHP1α States in Their KCl-Mediated Liquid-Liquid Phase Separation (LLPS) Using Solid-State NMR (SSNMR) and Molecular Dynamic (MD) Simulations
    Chin, Sze Yuet
    Chen, Yinglu
    Zhao, Lei
    Liu, Xinyi
    Chng, Choon-Peng
    Soman, Aghil
    Nordenskio''ld, Lars
    Huang, Changjin
    Shi, Xiangyan
    Xue, Kai
    JOURNAL OF PHYSICAL CHEMISTRY B, 2024, 128 (42): : 10451 - 10459
  • [2] Liquid-Liquid Phase Separation in Model Nuclear Waste Glasses: A Solid-State Double-Resonance NMR Study
    Martineau, Charlotte
    Michaelis, Vladimir K.
    Schuller, Sophie
    Kroeker, Scott
    CHEMISTRY OF MATERIALS, 2010, 22 (17) : 4896 - 4903
  • [3] Liquid-liquid phase coexistence in lipid membranes observed by natural abundance 1H-13C solid-state NMR
    Loeser, Lucas
    Saalwaechter, Kay
    Ferreira, Tiago Mendes
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2018, 20 (15) : 9751 - 9754
  • [4] Solution NMR spectroscopy for investigation of liquid-liquid phase separation
    Saio, Tomohide
    Okumura, Masaki
    Lee, Young-Ho
    JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY, 2020, 24 (02): : 47 - 52
  • [5] Molecular Forces in the Liquid-Liquid Phase Separation of Biomolecules
    Welsh, Timothy J.
    Krainer, Georg
    Knowles, Tuomas P.
    BIOPHYSICAL JOURNAL, 2020, 118 (03) : 373A - 373A
  • [6] Uncovering the molecular language of protein liquid-liquid phase separation
    Rekhi, Shiv
    Garcia, Cristobal
    Barai, Mayur
    Rizuan, Azamat
    Schuster, Benjamin S.
    Kiick, Kristi L.
    Mittal, Jeetain
    BIOPHYSICAL JOURNAL, 2024, 123 (03) : 444A - 445A
  • [7] Expanding the molecular language of protein liquid-liquid phase separation
    Rekhi, Shiv
    Garcia, Cristobal Garcia
    Barai, Mayur
    Rizuan, Azamat
    Schuster, Benjamin S.
    Kiick, Kristi L.
    Mittal, Jeetain
    NATURE CHEMISTRY, 2024, 16 (07) : 1113 - 1124
  • [8] Chemical Insights into Liquid-Liquid Phase Separation in Molecular Biology
    Kamimura, Yugo R.
    Kanai, Motomu
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 2021, 94 (03) : 1045 - 1058
  • [9] Solid-state NMR studies of molecular motion
    Duer, MJ
    ANNUAL REPORTS ON NMR SPECTROSCOPY, VOL 43, 2001, 43 : 1 - 58
  • [10] Solid-state NMR studies of molecular motion
    Duer, Melinda J.
    ANNUAL REPORTS ON NMR SPECTROSCOPY, VOL 59, 2006, 59 : 41 - 116