CRISPR-Based Editing of the Medicago truncatula LEC1 Gene

被引:0
|
作者
Potsenkovskaia, Elina A. [1 ,2 ,3 ]
Tvorogova, Varvara E. [1 ,2 ,3 ]
Simonova, Veronika Y. [2 ]
Konstantinov, Zakhar S. [1 ,2 ]
Kiseleva, Anna S. [2 ]
Matveenko, Andrew G. [1 ,2 ]
Brynchikova, Anna V. [2 ]
Lutova, Ludmila A. [1 ,2 ]
机构
[1] St Petersburg State Univ, Dept Genet & Biotechnol, 7-9 Univ Skaya Embankment, St Petersburg 199034, Russia
[2] Sirius Univ Sci & Technol, Plant Biol & Biotechnol Dept, 1 Olymp Ave, Soci 354340, Russia
[3] NI Vavilov All Russian Inst Plant Genet Resources, Ctr Genet Technol, 42 Bolshaya Morskaya St, St Petersburg 190000, Russia
来源
PLANTS-BASEL | 2024年 / 13卷 / 22期
关键词
CRISPR; LEC1; MtNF-YB10; <italic>Medicago truncatula</italic>; agrobacterial transformation; regeneration; somatic embryogenesis; SEED OIL PRODUCTION; REGULATORY NETWORKS; LEAFY COTYLEDON1; TRANSCRIPTION; TRANSFORMATION; MUTAGENESIS; IMPROVEMENT; EXPRESSION; INSIGHTS; ACID;
D O I
10.3390/plants13223226
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arabidopsis thaliana LEAFY COTYLEDON1 (LEC1) gene is shown to have numerous diverse functions in plant development, including the regulation of embryo morphogenesis and maturation, hypocotyl elongation, flowering transition, etc. However, the functions of LEC1 orthologs in different plant species have not been extensively studied. In this study, we obtained a line of Medicago truncatula, a model leguminous plant, carrying the loss-of-function mutation in the MtLEC1 (MtNF-YB10) gene, orthologous to LEC1, using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas9) genome editing system. Edited plants with loss of MtNF-YB10 function did not demonstrate any severe abnormalities during their normal growth and gave viable seeds, but their capability for somatic embryogenesis in vitro was dramatically reduced. The T1 progeny of unedited plants with a Cas9-gRNA cassette insertion was also analyzed based on the suggestion that editing could occur during seed formation. However, no edited plants were found in the T1 generation. These results suggest divergent functions of LEC1 orthologs and make it possible to investigate potential specific MtNF-YB10 functions.
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页数:15
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