A Magnetic Nanocarrier of Ciprofloxacin Used for Restraining the Growth of the Multidrug-Resistant Pseudomonas aeruginosa

被引：0

作者：

Giannousi, Kleoniki ^{[1
]}

Zouni, Eleni ^{[1
]}

Grigoriadis, Nikolaos ^{[2
,3
]}

Vizirianakis, Ioannis S. ^{[2
,4
]}

Oikonomou, Ilias M. ^{[5
,6
]}

Nicolosi, Valeria ^{[5
,6
]}

Dendrinou-Samara, Catherine ^{[1
]}

机构：

[1] Aristotle Univ Thessaloniki, Dept Chem, Lab Inorgan Chem, Thessaloniki 54124, Greece

[2] Aristotle Univ Thessaloniki, Sch Pharm, Lab Pharmacol, Thessaloniki 54124, Greece

[3] Biogenea Pharmaceut Ltd, LIMANI Ctr, 26th Oktovriou 43,5th Floor 5-06, Thessaloniki 54627, Greece

[4] Univ Nicosia, Sch Life & Hlth Sci, Dept Hlth Sci, CY-2417 Nicosia, Cyprus

[5] Trinity Coll Dublin, CRANN & AMBER Res Ctr, Dublin D02PN40, Ireland

[6] Trinity Coll Dublin, Sch Chem, Dublin D02PN40, Ireland

来源：

INORGANICS | 2025年 / 13卷 / 02期

关键词：

magnetic nanocarrier; <italic>Pseudomonas aeruginosa</italic>; multidrug resistance; NANOPARTICLES; COPPER;

D O I：

10.3390/inorganics13020058

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Ciprofloxacin (CPL) is an effective antibiotic against Pseudomonas aeruginosa. However, its use is limited by the emergence of multi-resistant strains. In this study, 8-15 nm manganese ferrite (MnFe2O4) nanoparticles, aminated and/or PEGylated, have been used as drug-delivery systems of CPL. The magnetic nanoparticles (MNPs) were prepared in the presence of the aliphatic amines octadecylamine (ODA), oleylamine (OAm), or PEG8000 to achieve the appropriate surface chemistry for the direct conjugation of CPL and drug loading into the PEG matrix, respectively. The primary MNPs proved to be biocompatible in calf thymus (CT)-DNA interaction studies, with binding constant values Kb in the range of 4.43-6.5 x 104 (g/mL)-1. ODA as a coater gave rise to MnFe2O4 MNPs, with a high percentage of free amines that further allowed for the conjugation of 90.9% CPL, which gradually released via a non-Fickian anomalous transport motif. The 25.1% CPL that loaded in the PEGylated MNPs led to a partial transformation of the nanoflowers into more aggregated forms. The release profile, although steeper, is described by the same model. The isolated magnetic nanocarrier with a high content of CPL was evaluated for its antimicrobial activity against a multi-resistant strain of P. aeruginosa using an automated industrial instrument (BacT/ALERT (R) 3D), and its molecular profile was outlined by studying its interaction with plasmid DNA (pDNA). The prototype use of BacT/ALERT (R) 3D allows for the simultaneous screening of multiple samples, while it foreshadows the transition to a preclinical phase.

引用

页数：19

共 46 条

[41] Estrella M.R., Brusseau M.L., Maier R.S., Pepper I.L., Wierenga P.J., Miller R.M., Biodegradation, sorption, and transport of 2,4-dichlorophenoxyacetic acid in saturated and unsaturated soils, Appl. Environ. Microbiol, 59, pp. 4266-4273, (1993)
[42] Hudaverdi A., Serpil G., Zelal I., Ozdemir S., Yalvac M., Dizge N., Deepanraj B., Ashraf G.A., Iron Oxide Nanoparticles Synthesis From Vermicomposting Leachate and its Antioxidant Activities, Front. Mater, 9, (2022)
[43] Varghese N., Jose J.R., Krishna P.M., Philip D., Joy F., Vinod T.P., Prathapachandra Kurup M.R., Nair Y., In vitro Analytical Techniques as Screening Tools to investigate the Metal chelate-DNA interactions, ChemistrySelect, 8, (2023)
[44] BacT/ALERT® 3D User Manual, (2010)
[45] Shahabadi N., Akbari A., Karampour F., Falsafi M., Zendehcheshm S., In vitro cytotoxicity, antibacterial activity and HSA and ct-DNA interaction studies of chlorogenic acid loaded on γ-Fe2O3@SiO2 as new nanoparticles, J. Biomol. Struct. Dyn, 41, pp. 2300-2320, (2023)
[46] Pasolli M., Dafnopoulos K., Andreou N.-P., Gritzapis P.S., Koffa M., Koumbis A.E., Psomas G., Fylaktakidou K.C., Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies, Molecules, 21, (2016)

← 1 2 3 4 5 →