Identification and Functional Analysis of the Ph-2 Gene Conferring Resistance to Late Blight (Phytophthora infestans) in Tomato

被引：0

作者：

Pan, Chunyang ^{[1
]}

Li, Xin ^{[1
]}

Lu, Xiaoxiao ^{[1
]}

Hu, Junling ^{[1
]}

Zhang, Chen ^{[1
]}

Shi, Lianfeng ^{[1
]}

Zhu, Can ^{[1
]}

Guo, Yanmei ^{[1
]}

Wang, Xiaoxuan ^{[1
]}

Huang, Zejun ^{[1
]}

Du, Yongchen ^{[1
]}

Liu, Lei ^{[1
]}

Li, Junming ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

来源：

PLANTS-BASEL | 2024年 / 13卷 / 24期

基金：

中国国家自然科学基金;

关键词：

tomato; late blight; <italic>Ph-2</italic>; candidate gene; hypersensitive responses; SOLANUM-PIMPINELLIFOLIUM; PLANT; PROTEIN; BACTERIAL; SPECIFICITY; DISCOVERY; GENOMICS; ENCODES; ALLELES; BINDING;

D O I：

10.3390/plants13243572

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene Ph-2 confers resistance against the race T1 of Phytophthora infestans in tomatoes. Herein, we identified Solyc10g085460 (RGA1) as a candidate gene for Ph-2 through the analysis of sequences and post-inoculation expression levels of genes located within the fine mapping interval. The RGA1 was subsequently validated to be a Ph-2 gene through targeted knockout and complementation analyses. It encodes a CC-NBS-LRR disease resistance protein, and transient expression assays conducted in the leaves of Nicotiana benthamiana indicate that Ph-2 is predominantly localized within the nucleus. In comparison to its susceptible allele (ph-2), the transient expression of Ph-2 can elicit hypersensitive responses (HR) in N. benthamiana, and subsequent investigations indicate that the structural integrity of the Ph-2 protein is likely a requirement for inducing HR in this species. Furthermore, ethylene and salicylic acid hormonal signaling pathways may mediate the transmission of the Ph-2 resistance signal, with PR1- and HR-related genes potentially involved in the Ph-2-mediated resistance. Our results could provide a theoretical foundation for the molecular breeding of tomato varieties resistant to late blight and offer valuable insights into elucidating the interaction mechanism between tomatoes and P. infestans.

引用

页数：17

共 73 条

[11] Hu C.H., Perez F.G., Donahoo R., McLeod A., Myers K., Ivors K., Secor G., Roberts P.D., Deahl K.L., Fry W.E., Et al., Recent Genotypes of Phytophthora infestans in the Eastern United States Reveal Clonal Populations and Reappearance of Mefenoxam Sensitivity, Plant Dis, 96, pp. 1323-1330, (2012)
[12] Ramadan A.A., Olfat M.M., Nour E.K.S., Kenta S., Said M.K., Mohamed T.R., Resistance to Phytophthora infestans in tomato wild relatives, Afr. J. Agric. Res, 12, pp. 2188-2196, (2017)
[13] Peirce L.C., A technique for screening Tomato plants for single gene resistance to race 0, Phytophthora infestans, Plant Dis. Report, 54, pp. 681-682, (1970)
[14] Brouwer D.J., Jones E.S., Clair D.A.S., QTL analysis of quantitative resistance to Phytophthora infestans (late blight) in tomato and comparisons with potato, Genome, 47, pp. 475-492, (2004)
[15] Chunwongse J., Chunwongse C., Black L., Hanson P., Molecular mapping of the Ph-3 gene for late blight resistance in tomato, J. Hortic. Sci. Biotechnol, 77, pp. 281-286, (2015)
[16] Zhang C., Liu L., Wang X., Vossen J., Li G., Li T., Zheng Z., Gao J., Guo Y., Visser R.G.F., Et al., The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans, Theor. Appl. Genet, 127, pp. 1353-1364, (2014)
[17] Lough R.C., Inheritance of Tomato Late Blight Resistance in Lycopersicon hirsutum LA1033, Ph.D. Dissertation, (2003)
[18] Sullenberger M.T., Jia M., Gao S., Ashrafi H., Foolad M.R., Identification of late blight resistance quantitative trait loci in Solanum pimpinellifolium accession PI 270441, Plant Genome, 15, (2022)
[19] Li J., Liu L., Bai Y., Finkers R., Wang F., Du Y., Yang Y., Xie B., Visser R.G.F., van Heusden A.W., Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777, Euphytica, 179, pp. 427-438, (2011)
[20] Brouwer D.J., St Clair D.A., Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILs) and sub-NILs, Theor. Appl. Genet, 108, pp. 628-638, (2004)

← 1 2 3 4 5 6 7 8 →