Identification and Functional Analysis of the Ph-2 Gene Conferring Resistance to Late Blight (Phytophthora infestans) in Tomato

被引：0

作者：

Pan, Chunyang ^{[1
]}

Li, Xin ^{[1
]}

Lu, Xiaoxiao ^{[1
]}

Hu, Junling ^{[1
]}

Zhang, Chen ^{[1
]}

Shi, Lianfeng ^{[1
]}

Zhu, Can ^{[1
]}

Guo, Yanmei ^{[1
]}

Wang, Xiaoxuan ^{[1
]}

Huang, Zejun ^{[1
]}

Du, Yongchen ^{[1
]}

Liu, Lei ^{[1
]}

Li, Junming ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

来源：

PLANTS-BASEL | 2024年 / 13卷 / 24期

基金：

中国国家自然科学基金;

关键词：

tomato; late blight; <italic>Ph-2</italic>; candidate gene; hypersensitive responses; SOLANUM-PIMPINELLIFOLIUM; PLANT; PROTEIN; BACTERIAL; SPECIFICITY; DISCOVERY; GENOMICS; ENCODES; ALLELES; BINDING;

D O I：

10.3390/plants13243572

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene Ph-2 confers resistance against the race T1 of Phytophthora infestans in tomatoes. Herein, we identified Solyc10g085460 (RGA1) as a candidate gene for Ph-2 through the analysis of sequences and post-inoculation expression levels of genes located within the fine mapping interval. The RGA1 was subsequently validated to be a Ph-2 gene through targeted knockout and complementation analyses. It encodes a CC-NBS-LRR disease resistance protein, and transient expression assays conducted in the leaves of Nicotiana benthamiana indicate that Ph-2 is predominantly localized within the nucleus. In comparison to its susceptible allele (ph-2), the transient expression of Ph-2 can elicit hypersensitive responses (HR) in N. benthamiana, and subsequent investigations indicate that the structural integrity of the Ph-2 protein is likely a requirement for inducing HR in this species. Furthermore, ethylene and salicylic acid hormonal signaling pathways may mediate the transmission of the Ph-2 resistance signal, with PR1- and HR-related genes potentially involved in the Ph-2-mediated resistance. Our results could provide a theoretical foundation for the molecular breeding of tomato varieties resistant to late blight and offer valuable insights into elucidating the interaction mechanism between tomatoes and P. infestans.

引用

页数：17

共 73 条

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