Objective: This study aims to examine the prevalence and risk factors related to VD deficiency among female adolescents. Methods: This cross-sectional study was done in secondary and preparatory schools in Sulaimaniyah, Iraq, from May 2022 to July 2023. A total of 120 schools were included in the study, with a calculated sample size of 340 female adolescents using a probability multistage random sampling method. Data was collected through a validated questionnaire, and blood samples were analyzed forVD levels. Results: In a study of 340 female adolescents aged 14-18 years, the findings showed that 39.4% of participants had severe VD deficiency, 42.6% were deficient, and 17.9% had insufficiency. The mean VD level was 13.29 +/- 6.45 ng/mL. Seasonal variation significantly impacted VD level (P = 0.001), with the highest levels seen in summer and the lowest in winter. Skin colour also significantly influenced VD level (P = 0.020), as individuals with lighter skin have higher levels.There were positive correlations between VD levels, milk intake (r = 0.138, P = 0.011), and cheese consumption (r = 0.135, P = 0.013). The use of sunscreen had mixed effects. Interestingly, there was a positive correlation between SPF 15 and VD level (r = 0.132, P = 0.015), while higher SPF values were associated with lower levels. Conclusion: The findings highlight the importance of seasonal adjustments, improved dietary habits, and lifestyle changes to address VD deficiency in the population effectively.