Taurine Inhibits Lung Metastasis in Triple-Negative Breast Cancer by Modulating Macrophage Polarization Through PTEN-PI3K/Akt/mTOR Pathway

被引:7
作者
Lin, Yufeng [1 ]
Huang, Yongtong [1 ]
Zheng, Yifan [1 ]
Chen, Wanting [2 ]
Zhang, Yongcheng [1 ]
Yang, Yongxia [2 ]
Huang, Wenbin [1 ,3 ]
机构
[1] Guangdong Pharmaceut Univ, Affiliated Hosp 1, Dept Breast Care Surg, Guangzhou, Peoples R China
[2] Guangdong Pharmaceut Univ, Sch Med Informat Engn, Guangzhou, Peoples R China
[3] Southern Med Univ, Zhujiang Hosp, Dept Hepatobiliary Surg 2, Guangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
Triple-negative breast cancer; Taurine; Tumor-associated macrophages; metastasis;
D O I
10.1097/CJI.0000000000000518
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
:Taurine (Tau) has been found to inhibit triple-negative breast cancer (TNBC) invasion and metastasis. However, its effect on tumor-promoting macrophages and tumor suppressor macrophages in breast cancer progression remains unknown. In this study, we investigated the effects of Tau on macrophage polarization and its role in TNBC cell growth, invasion, and metastasis. We induced human THP-1 monocytes to differentiate into M2 macrophages through exogenous addition of interleukin-4. We used the TNBC cell lines MDA-MB-231 and BT-549 cultured in a conditioned medium from M2 macrophages to investigate the effect of Tau on tumor growth and invasion. We analyzed macrophage subset distribution, M1 and M2 macrophage-associated markers, and mRNA expression by quantitative polymerase chain reaction. We also detected the PTEN-PI3K/Akt/mTOR signaling pathway that mediates M1 macrophage to suppress tumor invasion using western blotting. Our results showed that Tau inhibits breast cancer metastasis to the lungs in vivo and cell invasion by altering the polarization of tumor-associated macrophage in vitro. In addition, Tau can up-regulate PTEN expression, suppress the PI3K-Akt signaling pathway, and promote the M1 polarization of macrophages, which ultimately inhibits the metastasis of TNBC cells. Our findings suggest that Tau inhibits the activation of the PI3K-Akt-mTOR signaling pathway by up-regulating PTEN, promotes the proportion of M1 macrophages in tumor-associated macrophage, and suppresses the invasion and metastasis of TNBC. This provides a potential therapeutic approach to influence cancer progression and metastasis.
引用
收藏
页码:369 / 377
页数:9
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