Laser-Assisted Micropatterned 3D Printed Scaffolds with Customizable Surface Topography and Porosity for Modulation of Cell Function

被引:0
|
作者
Aboal-Castro, Lucia [1 ,2 ]
Radziunas-Salinas, Yago [2 ,3 ,4 ]
Pita-Vilar, Maria [1 ,2 ]
Carnero, Bastian [2 ,3 ,4 ]
Mikos, Antonios G. [5 ]
Alvarez-Lorenzo, Carmen [1 ,2 ]
Flores-Arias, Maria Teresa [2 ,3 ]
Diaz-Gomez, Luis [1 ,2 ]
机构
[1] Univ Santiago de Compostela, Inst Santiago De Compostela IDIS, Fac Farm & Hlth Res, Dept Pharmacol Pharm & Pharmaceut Technol,I D Farm, Santiago De Compostela 15782, Spain
[2] Univ Santiago de Compostela, Inst Mat iMATUS, Santiago De Compostela 15782, Spain
[3] Univ Santiago de Compostela, Appl Phys Dept, Photon 4 Life Res Grp, Fac Fis, Campus Vida, Santiago De Compostela 15782, Spain
[4] Univ Santiago de Compostela, Fac Opt Optometria, Campus Vida, Santiago De Compostela 15782, Spain
[5] Rice Univ, Dept Bioengn, Houston, TX 77030 USA
关键词
biofabrication; femtosecond laser engraving; micropatterns; tissue engineering; topographies; FEMTOSECOND LASER; BONE TISSUE; FABRICATION; ABLATION; MATRIX; PCL;
D O I
10.1002/adhm.202403992
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The dynamic interaction between cells and their substrate is a cornerstone of biomaterial-based tissue regeneration focused on unraveling the complex factors that govern this crucial relationship. A key challenge is translating physical cues from 2D to 3D due to limitations in current biofabrication techniques. In response, this study introduces an innovative approach that combines additive and subtractive manufacturing for precise surface patterning of 3D printed scaffolds. Using poly(epsilon-caprolactone) as the scaffold material, polymeric fibers are 3D printed and subsequently laser-engraved with femtosecond laser to precisely create controlled microtopographies, including microgrooves (10 and 80 mu m in width) and micropits (25 mu m in diameter). Testing shows that the process does not compromise the mechanical properties of the fibers, which is critical for structural applications in tissue engineering. Human mesenchymal stem cells are used to investigate the effects of these topographical features on cell behavior. The 10 mu m wide microgrooves notably enhance cell attachment, with cells aligning in elongated forms along the grooves, while micropits and unpatterned surfaces promote polygonal cell shapes. This combined approach demonstrates that precisely engineered microtopographies on 3D printed scaffolds can better mimic the natural extracellular matrix, improving cellular responses and offering a promising strategy for advancing tissue regeneration.
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页数:13
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