Transcriptomic Analysis of Air-Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation

被引：0

作者：

Kim, Jieun ^{[1
,2
]}

Eo, Eun-Young ^{[1
]}

Kim, Bokyong ^{[1
]}

Lee, Heetak ^{[3
]}

Kim, Jihoon ^{[3
,4
]}

Koo, Bon-Kyoung ^{[3
]}

Kim, Hyung-Jun ^{[1
]}

Cho, Sukki ^{[5
]}

Kim, Jinho ^{[6
,7
,8
]}

Cho, Young-Jae ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Internal Med, Div Pulm & Crit Care Med,Bundang Hosp, Seongnam 13620, South Korea

[2] CHA Univ, Dept Biomed Sci, Seongnam 13488, South Korea

[3] Inst for Basic Sci Korea, Ctr Genome Engn, Daejeon 34126, South Korea

[4] Catholic Univ Korea, Dept Med & Biol Sci, Bucheon 14662, South Korea

[5] Seoul Natl Univ, Bundang Hosp, Dept Thorac & Cardiovasc Surg, Seongnam 13620, South Korea

[6] Seoul Natl Univ, Bundang Hosp, Dept Genom Med, Seongnam 13620, South Korea

[7] Seoul Natl Univ, Precis Med Ctr, Future Innovat Res Div, Bundang Hosp, Seongnam 13620, South Korea

[8] Seoul Natl Univ, Bundang Hosp, Coll Med, Dept Lab Med, Seongnam 13620, South Korea

来源：

CELLS | 2024年 / 13卷 / 23期

基金：

新加坡国家研究基金会;

关键词：

airway epithelium; air-liquid interface; ciliated cells; differentiation; single-cell RNA-sequencing; STEM-CELLS; PROMOTES; CYCLE;

D O I：

10.3390/cells13231991

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To develop in vitro respiratory models, it is crucial to identify the factors involved in epithelial cell differentiation. In this study, we comprehensively analyzed the effects of air-liquid interface (ALI) culture on epithelial cell differentiation using single-cell RNA sequencing (scRNA-seq). ALI culture induced a pronounced shift in cell composition, marked by a fivefold increase in ciliated cells and a reduction of more than half in basal cells. Transcriptional signatures associated with epithelial cell differentiation, analyzed using iPathwayGuide software, revealed the downregulation of VEGFA and upregulation of CDKN1A as key signals for epithelial differentiation. Our findings highlight the efficacy of the ALI culture for replicating the human lung airway epithelium and provide valuable insights into the crucial factors that influence human ciliated cell differentiation.

引用

页数：17

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