Evaluation of the Eazyplex® Candida ID LAMP Assay for the Rapid Diagnosis of Positive Blood Cultures

被引：1
|
作者：
Berlau, Arvid [1 ]
Stoll, Sylvia [1 ]
Edel, Birgit [1 ]
Loeffler, Bettina [1 ]
Roedel, Juergen [1 ]
机构：
[1] Friedrich Schiller Univ, Jena Univ Hosp, Inst Med Microbiol, D-07747 Jena, Germany
来源：
DIAGNOSTICS | 2024年 / 14卷 / 19期
关键词：
Candida; sepsis; blood culture; rapid diagnostic test; LAMP; ESCMID-ASTERISK GUIDELINE; MANAGEMENT; IDENTIFICATION; ADULTS;
D O I：
10.3390/diagnostics14192125
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Rapid molecular assays can be used to identify Candida pathogens directly from positive blood cultures (BCs) in a timely manner compared to standard methods using subcultures. In this study, the eazyplex (R) Candida ID assay, which is based on loop-mediated amplification (LAMP) and is currently for research use only, was evaluated for the identification of the most common fungal species. A total of 190 BCs were analysed. Sensitivity and specificity were 93.88% and 99.26% for C. albicans, 89.13% and 100% for Nakaseomyces glabratus (N. glabratus), 100% and 100% for Pichia kudravzevii (P. kudriavzevii), 100% and 100% for C. tropicalis, and 100% and 99.44% for C. parapsilosis. Sample preparation took approximately 11 min and positive amplification results were obtained between 8.5 and 19 min. The eazyplex (R) Candida ID LAMP assay is an easy-to-use diagnostic tool that can optimise the management of patients with candidemia.
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页数：11
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