Evaluation of the Eazyplex® Candida ID LAMP Assay for the Rapid Diagnosis of Positive Blood Cultures

被引：1
|
作者：
Berlau, Arvid [1 ]
Stoll, Sylvia [1 ]
Edel, Birgit [1 ]
Loeffler, Bettina [1 ]
Roedel, Juergen [1 ]
机构：
[1] Friedrich Schiller Univ, Jena Univ Hosp, Inst Med Microbiol, D-07747 Jena, Germany
来源：
DIAGNOSTICS | 2024年 / 14卷 / 19期
关键词：
Candida; sepsis; blood culture; rapid diagnostic test; LAMP; ESCMID-ASTERISK GUIDELINE; MANAGEMENT; IDENTIFICATION; ADULTS;
D O I：
10.3390/diagnostics14192125
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Rapid molecular assays can be used to identify Candida pathogens directly from positive blood cultures (BCs) in a timely manner compared to standard methods using subcultures. In this study, the eazyplex (R) Candida ID assay, which is based on loop-mediated amplification (LAMP) and is currently for research use only, was evaluated for the identification of the most common fungal species. A total of 190 BCs were analysed. Sensitivity and specificity were 93.88% and 99.26% for C. albicans, 89.13% and 100% for Nakaseomyces glabratus (N. glabratus), 100% and 100% for Pichia kudravzevii (P. kudriavzevii), 100% and 100% for C. tropicalis, and 100% and 99.44% for C. parapsilosis. Sample preparation took approximately 11 min and positive amplification results were obtained between 8.5 and 19 min. The eazyplex (R) Candida ID LAMP assay is an easy-to-use diagnostic tool that can optimise the management of patients with candidemia.
引用
收藏
页数：11
相关论文
共 50 条
  • [1] The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures
    Sekowska, Alicja
    Bogiel, Tomasz
    ANTIBIOTICS-BASEL, 2022, 11 (02):
  • [2] Evaluation of Eazyplex® LAMP test for fast Candida auris direct detection of colonized patients
    Felices, Francisco Javier Hernandez
    Palop, Nuria Tormo
    Garcia, Carme Salvador
    Bayona, Juan Vicente Mulet
    Serrano, M. Remedio Guna
    Cardona, Concepcion Gimeno
    MYCOSES, 2024, 67 (01)
  • [3] Rapid diagnosis of false positive blood cultures in newborns
    Kelley, RN
    PEDIATRIC RESEARCH, 2003, 53 (04) : 484A - 484A
  • [4] Assessment of a Multiplex LAMP Assay (Eazyplex(R) CSF Direct M) for Rapid Molecular Diagnosis of Bacterial Meningitis: Accuracy and Pitfalls
    Leroy, Anne-Gaelle
    Persyn, Elise
    Gibaud, Sophie-Anne
    Cremet, Lise
    Le Turnier, Paul
    Benhamida, Myriam
    Launay, Elise
    Guillouzouic, Aurelie
    Bemer, Pascale
    Corvec, Stephane
    MICROORGANISMS, 2021, 9 (09)
  • [5] Evaluation of the Amplex eazyplex Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Pneumocystis jirovecii Pneumonia
    Huber, Timo
    Serr, Annerose
    Geissdoerfer, Walter
    Hess, Christina
    Lynker-Assmus, Christian
    von Loewenich, Friederike D.
    Bogdan, Christian
    Held, Juergen
    JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (12)
  • [6] Evaluation of rapid colistin susceptibility directly from positive blood cultures using a flow cytometry assay
    Fonseca e Silva, D.
    Silva-Dias, A.
    Gomes, R.
    Martins-Oliveira, I
    Ramos, M. H.
    Rodrigues, A. G.
    Canton, R.
    Pina-Vaz, C.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2019, 54 (06) : 820 - 823
  • [7] Rapid detection of β-lactamases directly from positive blood cultures using a loop-mediated isothermal amplification (LAMP)-based assay
    Zboromyrska, Yuliya
    Vergara, Andrea
    Cosgaya, Clara
    Verger, Gabriel
    Mosqueda, Noraida
    Almela, Manel
    Pitart, Cristina
    Roca, Ignasi
    Marco, Francesc
    Vila, Jordi
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2015, 46 (03) : 355 - 356
  • [8] Evaluation of a Microarray-Based Assay for Rapid Identification of Gram-Positive Organisms and Resistance Markers in Positive Blood Cultures
    Samuel, Linoj P.
    Tibbetts, Robert J.
    Agotesku, Adam
    Fey, Margaret
    Hensley, Rhonda
    Meier, Frederick A.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (04) : 1188 - 1192
  • [9] Comparison of PneumID real-time PCR assay with Amplex eazyplex LAMP assay for laboratory diagnosis of Pneumocystis jirovecii Pneumonia
    Ng, Willy W. Y.
    Ho, Yolanda I. I.
    Wong, Ann H.
    Leung, Eddie C. M.
    Lee, Alfred L. H.
    Chow, Viola C. Y.
    MEDICAL MYCOLOGY, 2022, 60 (06)
  • [10] VALUE OF THE EAZYPLEX® CSF DIRECT ASSAY IN RAPID DIAGNOSIS OF INVASIVE MENINGOCOCCAL DISEASE - CASE REPORT
    Karrasch, Matthias
    Eisenach, Sven
    Vogel, Ulrich
    Zinke, Jan
    Witte, Otto W.
    Guenther, Albrecht
    Romeike, Bernd
    Roedel, Juergen
    ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 2018, 65 (03) : 309 - 315
12345