Poor reproducibility of percentage of normally shaped sperm using the World Health Organization Fifth Edition strict grading criteria

被引：7

作者：

Baker, Karen C. ^{[1
]}

Steiner, Anne Z. ^{[2
]}

Hansen, Karl R. ^{[3
]}

Barnhart, Kurt T. ^{[4
]}

Cedars, Marcelle I. ^{[5
]}

Legro, Richard S. ^{[6
]}

Diamond, Michael P. ^{[7
]}

Krawetz, Stephen A. ^{[8
,9
]}

Usadi, Rebecca ^{[10
]}

Baker, Valerie L. ^{[11
]}

Coward, R. Matthew ^{[12
]}

Sun, Fangbai ^{[13
]}

Wild, Robert ^{[14
]}

Masson, Puneet ^{[15
]}

Smith, James F. ^{[16
]}

Santoro, Nanette ^{[17
]}

Zhang, Heping ^{[18
]}

机构：

[1] Duke Univ, Div Urol, Durham, NC USA

[2] Duke Univ, Dept Obstet & Gynecol, Durham, NC USA

[3] Univ Oklahoma, Hlth Sci Ctr, Dept Obstet & Gynecol, Oklahoma City, OK USA

[4] Univ Penn, Dept Obstet & Gynecol, Philadelphia, PA USA

[5] Univ Calif San Francisco, Dept Obstet & Gynecol, San Francisco, CA USA

[6] Penn State Univ, Dept Obstet & Gynecol, Hershey, PA USA

[7] Augusta Univ, Dept Obstet & Gynecol, Med Coll Georgia, Augusta, GA USA

[8] Wayne State Univ, Dept Obstet & Gynecol, Detroit, MI USA

[9] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI USA

[10] Atrium Hlth, Dept Reprod Endocrinol & Infertil, Charlotte, NC USA

[11] Johns Hopkins Univ, Dept Gynecol & Obstet, Lutherville Timonium, MD USA

[12] Univ N Carolina, Dept Urol, Chapel Hill, NC USA

[13] Yale Sch Publ Hlth, Dept Biostat, New Haven, CT USA

[14] Univ Oklahoma, Hlth Sci Ctr, Dept Obstet & Gynecol, Hlth Sci Ctr, Oklahoma City, OK 73190 USA

[15] Univ Penn, Dept Urol, Philadelphia, PA USA

[16] Univ Calif San Francisco, Dept Urol, San Francisco, CA USA

[17] Univ Colorado, Dept Obstet & Gynecol, Aurora, CO USA

[18] Yale Sch Publ Hlth, Collaborat Ctr Stat Sci, New Haven, CT USA

来源：

F&S REPORTS | 2022年 / 3卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Semen analysis; teratozoospermia; spermatozoa; quality control; male factor infertility; SEMEN QUALITY; PREDICTIVE-VALUE; MORPHOLOGY; FERTILE; VARIABILITY; PREGNANCY; PARAMETERS; EXPERIENCE; IMPACT; MEN;

D O I：

10.1016/j.xfre.2022.03.003

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To determine the reproducibility of the World Health Organization Fifth Edition (WHO5) strict grading methodology by comparing the percentage of morphologically normal sperm (PNS) recorded by the core laboratory with results obtained at the fertility centers participating in a multisite clinical trial. Design: Secondary cohort analysis of data from the Males, Antioxidants, and Infertility trial. Setting: Fertility centers. Patient(s): Semen values of 171 men participating in a multicenter, double-blind, randomized, placebo-controlled trial evaluating the effect of antioxidants on male fertility. Intervention(s): Not applicable. Main Outcome Measure(s): Strict morphology expressed as PNS as determined at each fertility center and the core central laboratory for the same semen sample. Result(s): No correlation was found in the PNS values for the same semen sample between the core laboratory and fertility center laboratories either as a group or by individual site. Interobserver agreement was similarly low (K- 0.05 and 0.15) between the core and fertility laboratories as a group for strict morphology, categorized by the WHO5 lower reference limits of 4% and 0, respectively. Moderate agreement was found between the core and 2 individual fertility laboratories for the cutoff value of 0 (K- 0.42 and 0.57). The remainder of the comparisons demonstrated poor to fair agreement. Conclusion(s): Strict morphology grading using the WHO5 methodology demonstrated overall poor reproducibility among a cohort of experienced fertility laboratories. This lack of correlation and agreement in the PNS values calls into question the reproducibility, and thereby the potential applicability, of sperm strict morphology testing. (Fertil Steril Rep (R) 2022;3:110-5. (c) 2022 by American Society for Reproductive Medicine.)

引用

页码：110 / 115

页数：6

共 27 条

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